Centre National De La Recherche Scientifique

Université Des Sciences Et Technologies De Lille

Eurogentec S.a.

Universite De Sciences Et Techologies De Lille

Université De Lille

Isabelle Fournier

Michel Salzet

Jean-Claude Edmond Tabet

Benoit Fatou

Gottfried Proess

Marc Lemaitre

Rémi Lemaire

Ivo Rudloff

Maxence Wisztorski

Rémi LeMaire

Cristian Focsa

Michael Ziskind

Michel Dechamps

Edmond Jean-Claude Tabet

Benoît Fatou

Michel Deschamps

Remi Lemaire

The invention relates to a biological material molecular analysis device characterized in that it includes:&#x2014;a laser () optionally using an optical parametric oscillator (OPO), configured to emit a wavelength between 2.5 ym and 12 put, said configured laser () being intended to ablate said biological material by ejecting charged and/or uncharged particles;&#x2014;a mass spectrometer (); and&#x2014;a probe (S,) comprising at least one first analysis fiber (A,), connected to the laser (), and a transfer tube (T,), connected to the mass spectrometer ().

Device for real-time in vivo molecular analysis

The invention concerns a method for determining at least one target molecule map in a tissue section, using at least one (A-X)n-B conjugate, wherein A is a tag molecule of known molecular weight, X is a linker that is cleaved during sample desorption/ionization, n is an integer of at least 1, and B is a binding molecule that binds specifically to said target molecule. When using MALDI mass spectrometry, said linker molecule X may be cleaved by photodissociation during sample laser irradiation if photocleavable at the wavelength of said MALDI laser. Alternatively, when using UV-MALDI, IR-MALDI, SIMS or DESI mass spectrometry, said linker molecule X may be cleaved by fragmentation during sample desorption/ionization.

Use of conjugates with linkers cleavable by photodissociation or fragmentation for mass spectrometry analysis of tissue sections

The invention concerns improved methods for studying peptides/proteins expression in a tissue section or for determining at least one compound, in particular a protein, expression map in a tissue section, using ionic MALDI matrices.

Use of ionic matrices for maldi mass spectrometry analysis of tissue sections

Growing community of inventors

Bourghelles, France

Isabelle Fournier