Uti Limited Partnership

Isabelle Barrette-Ng

Sui-Lam Wong

Sau-Ching Wu

Ken Ng

Kenneth K-s Ng

The present invention relates to a new streptavidin muteins. This mutein is binds both streptavidin binding peptide tagged and biotin or biotinylated molecules, and does so in a reversible fashion. As such, it is stable enough to allow reuse, and producible with reasonable production yield via secretion in a soluble functional state without the requirement of refolding via the tedious and expensive denaturation and renaturation processes.

Streptavidin mutein exhibiting reversible binding for biotin and streptavidin binding peptide tagged proteins

The present invention relates to a new streptavidin muteins. This mutein is The muteins are capable of oligomerization to form tetramers, with relatively strong subunit interactions, dissociation constant (K) for biotin in this mutein in the range of 10to 10M, off-rate (k) for the bound biotin in the streptavidin-biotin complex in the range of 10sec, stable enough to allow reuse, and producible producable with reasonable production yield via secretion in a soluble functional state without the requirement of refolding streptavidin via the tedious and expensive denaturation and renaturation processes.

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Calgary, Canada

Isabelle Barrette-Ng