Eppendorf Ag

Eppendorf Array Technologies S.a.

Eppendorf Array Technologies Sa (eat)

Eppendorf Array Technologies

Eppendorf Array Technologies (e.a.t.)

Isabelle Alexandre

José Remacle

Nathalie Zammatteo

Dieter Husar

Heinz Koehn

Sylvain Margaine

Sandrine Hamels

Francoise De Longueville

Sven De Roeck

Sophie Dufour

Laurence Lockman

Joseph Demarteau

Yves Houbion

Sven Buelow

Wilhelm Pluester

Christelle Plennevaux

Sylvian Margaine

Nathalie Zammateo

The present invention relates to a method and device for detecting and/or quantifying one or multiple target molecules present in a solution by quantifying online their binding on specific capture molecules immobilized at different locations (spots) of a surface of an optically transparent solid support without substantial detection of target molecules present in solution. The present invention allows multiple target assays to be performed in a simultaneous detection. More particularly, the invention comprises detecting in real-time the hybridization between capture DNA molecules present on a micro-array and target polynucleotides present in solution. The invention is also related to real-time PCR of multiple targets on a micro-array.

Detection and/or quantification method of target molecules on a solid support

The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (), a support () having fixed upon its surface a micro-array comprising at least 5 capture molecules () being immobilized in specifically localized areas () of said support; introducing said labeled target molecules solution () into the chamber; incubating said labeled target molecules under stable and controlled temperature conditions to allow the binding between said target and capture molecules; directing an excitation light () from a light source () on the surface of the micro-array; measuring the electromagnetic light emission () from the bound target molecules in response to said excitation light in presence of the solution containing the target molecules wherein the surface of emission for a localized area is comprised between about 0.1 &#x3bc;mand about 10 mmand wherein each of the at least 4 localized areas is monitored with time with at least two measurements being done per localized area (), and processing and storing the values of the different measurements and quantifying at least 4 different target molecules present in solution using at least one measurement value for each said target.

Real-time quantification of multiple targets on a micro-array

The present invention relates to a method and apparatus for monitoring on a micro-array a PCR amplification of a nucleotide molecule being present in a solution. The method includes the steps of: providing a support having fixed upon its surface a microarray having at least a capture molecule being immobilized in specifically localized areas of the support and a reaction chamber; introducing a solution containing the nucleotide molecule into the reaction chamber and reagents for nucleotide molecule amplification and labelling; submitting the solution to at least 2 thermal cycles having at least 2 and preferably 3 different temperature steps in order to obtain labelled target nucleotide molecule by PCR amplification; performing at least a measurement of the labelled target nucleotide molecule in at least one thermal cycle by incubating the labelled target nucleotide molecule under conditions allowing a specific binding between the target nucleotide molecule and its corresponding capture molecule and measuring the light emission from the bound labelled target nucleotide molecule in response to excitation light with the solution being present in the chamber and containing the labelled target nucleotide molecule. The surface of emission for a localized area is between about 0.1 &#x3bc;mand about 75 mm. The method further includes processing the data obtained in at least one thermal cycle in order to detect and/or quantify the amount of nucleotide molecule present in the solution before the amplification.

Real-time PCR of targets on a micro-array

Disclosed is a system and method conducting real-time PCR. Unlabeled capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.

Identification and quantification of a plurality of biological (micro)organisms or their components

Disclosed is a system and method conducting real-time PCR. Capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In an alternate embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.

The present invention is related to a method for the identification and/or the quantification of a target compound obtained from a sample, preferably a biological sample, comprising the steps of putting into contact the target compound with a capture molecule in order to allow a specific binding between the target compound with a capture molecule, the capture molecule being fixed upon a surface of a solid support according to an array comprising a density of at least 20 discrete regions per cm, each of the discrete regions being fixed with one species of capture molecules, performing a reaction leading to a precipitate formed at the location of the binding, determining the possible presence of precipitate(s) in discrete region(s), and correlating the presence of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of the target compound.

Method for the identification and/or the quantification of a target compound obtained from a biological sample upon chips

A method for identifying or quantifying an organism by a detecting its nucleotide sequence among at least 4 other homologous sequences comprising amplifying nucleic acids from the organism to generate target nucleotide sequences to be detected; contacting the target nucleotide sequences with single stranded capture nucleotide sequences bound by a single predetermined link to an insoluble solid support and discriminating the binding of a target nucleotide sequence specific of an organism with a signal resulting from a hybridization by complementary base pairing between the target nucleotide sequence and its corresponding capture nucleotide sequence is disclosed. The capture nucleotide sequence is bound to the insoluble solid support at a specific location on an array having a density of at least 4 different bound single stranded capture nucleotide sequences/cm. The location of the signal on the array allows identification or quantification of the organism.

Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays

The present invention is related to an identification and/or quantification method of a large number of biological organisms groups at different levels (family, genus, species) or part of those (possibly present in a biological sample) by a detection of their nucleotide sequence.

Growing community of inventors

Haltinne, Belgium

Isabelle Alexandre