Inscripta, Inc.

Isaac David Wagner

Phillip Belgrader

Eileen Spindler

Miles Gander

Matthew David Estes

Clint Davis

Krishna Yerramsetty

Paul Hardenbol

Thomas Hraha

Shea Lambert

Chad Douglas Sanada

Julia Swavola

Andrea Halweg-Edwards

The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

Detection of nuclease edited sequences in automated modules and instruments

Disclosed systems and methods relate to predicting the relative representation of genomic variants in an edited cell population, based on the editing cassette design representation in an editing cassette design library used to generate the edited cell population. A library of editing cassette designs is generated, and a feature vector, or sequence embedding, is developed for each design using natural language processing techniques. The feature vector may be based upon sequence attributes and editing kinetics of each cassette design as well as attributes that describe the library context. Features may include sequence embeddings generated from a neural network, linguistic-type distances, and statistical distance summaries thereof. The feature vectors are classified using one or more machine learning models, and the classified feature vectors are used to predict the representation of each design an edited cell population.

Methods and systems for modeling of design representation in a library of editing cassettes

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Longmont, CO, United States of America

Isaac David Wagner