University of Michigan

The Procter Gamble Company

Genencor International, Inc.

Other

Irwin J Goldstein

Harry Winter

Ann Margaret Wolff

Pushkaraj J Lad

David M Markovitz

Richard S Carpenter

Michael Swanson

Leslie A Riblet

Stephen J Rinderle

Inigo S De Tejada

Robert P Kruger

The present invention relates to chemical compounds, methods for their discovery, and their therapeutic and research use. In particular, the present invention provides antiviral and antimicrobial lectin compounds and methods of their use.

Lectins and uses thereof

The present invention relates to chemical compounds, methods for their discovery, and their therapeutic and research use. In particular, the present invention provides antiviral and antimicrobial lectin compounds (BanLec) isolated from bananas. These compounds have low mitogenicity and pro-inflammatory activity while maintaining anti-HIV-1 activity.

The isolation, characterization, cloning and expression of the lectin (agglutinin) from(MOA) is described. MOA displays unique carbohydrate binding properties, including blood group B-specific agglutination and preferential binding to Ga1&#x3b1;1,3Ga1-containing sugar epitopes, including but not limited to Ga1&#x3b1;1,3Ga1&#x3b2;1,4G1cNAc. MOA is contemplated as an affinity reagent, a therapeutic in the treatment of antibiotic-induced diarrhea and the field of xenotransplantation. MOA may also serve as a diagnostic reagent, e.g. for malaria.

Isolation, characterization, cloning and use of a mushroom lectin

Amaranthin, a new lectin isolated from the seeds of Amaranthus caudatus, is purified and characterized. Amaranthin, designated ACA (Amaranthus caudatus agglutinin), has a high affinity for the T-antigen glycoconjugate and variants thereof expressed by proliferating colorectal epithelium. ACA selectively binds glycoconjugates in the lower half of the colonic crypt and is useful as a marker of colonic epithelial cell differentiation in the human colon. ACA also binds to glycoconjugates in neoplastic colonic tissues, and binds to preneoplastic polypoid and flat colonic tissue from patients with familial polyposis coli. ACA is also readily adapted for quantitative measurement of lectin-binding sites. ACA binding assays can thus be used for detecting early stage abnormalities of proliferation and differentiation in normal-appearing preneoplastic colorectal epithelium and to diagnose neoplastic premalignant and malignant colorectal lesions.

Purified lectin and methods of use

A cleaning composition is disclosed. The composition contains a first enzyme where the enzyme can be Endo-D, Endo-H, Endo-L, Endo-C, Endo-CII, Endo-F-Gal type, Endo-F and PNGaseF and a second enzyme where the enzyme can be a protease, lipase, nuclease, glycosidase, an enzyme different from the first enzyme, and any combination of these. The composition also contains a detergent surfactant and a builder. The composition can be used in a method for cleaning a surface on which is bound a glycoside-containing substance. The substance can be blood or components thereof, fecal matter or components thereof or microorganisms. The surface can be fabric, biological tissue, tooth enamel, contact lens, glass, ceramic, metal, metal alloy, plastic, plant, fruit and vegetable.

Cleaning composition containing a type II endoglycosidase

A method for cleaning a surface on which is bound a glycoside-containing substance. The substance can be blood or components thereof, fecal matter or components thereof or microorganisms. The surface can be fabric, biological tissue, tooth enamel, contact lens, glass, ceramic, metal, metal alloy, plastic, plant, fruit and vegetable. A Type II endoglycosidase is used to carry out the method.

Method for cleaning a surface on which is bound a glycoside-containing

Etoperidone and its pharmaceutically acceptable salts are useful in the treatment of male sexual impotence.

Growing community of inventors

Ann Arbor, MI, United States of America

Irwin J Goldstein