Meloy Laboratories Inc.

Irwin A Braude

Disclosed is a process for the purification of crude immune interferon to a near homogeneous preparation which comprises: (a) adsorbing the crude interferon onto a column containing Controlled Pore Glass beads and eluting with ammonium sulfate, (b) adsorbing the interferon containing eluant onto a column containing either Concanavalin A-Sepharose, lentil lectin-Sepharose or pea lectin-agarose and eluting with a buffer containing a sugar, (c) adsorbing the interferon containing eluant onto a column containing Heparin-Sepharose or Procian Red-agarose and eluting with a high salt content buffer, (d) adsorbing the interferon containing eluant onto a cationic exchange resin column and eluting with a salt buffer and (e) treating the interferon containing eluant in a gel-filtration column equilibrated in high salt to obtain a solution of immune interferon that is nearly homogeneous.

Human immune interferon

Disclosed is a process for the purification of crude immune interferon to a near homogeneous preparation which comprises: (a) adsorbing the crude interferon onto a column containing Controlled Pore Glass beads and eluting with ammonium sulfate, (b) adsorbing the interferon containing eluant onto a column containing either Concanavalin A-Sepharose, lentil lectin-Sepharose or pea lectin-agarose and eluting with a buffer containing a sugar, (c) adsorbing the interferon containing eluant onto a column containing Heparin-Sepharose or Procian Red-agarose and eluting with a high salt content buffer, and (d) treating the interferon containing eluant in a gel-filtration column equilibrated in high salt to obtain a solution of immune interferon that is nearly homogeneous.

Purification of human immune interferon

A process is disclosed for producing human immune interferon with cell cultures of human peripheral blood leukocytes modulated with Mezerein or 12,13 phorbol dibutyrate and induced with Staphylococcal aureus enterotoxin B. After cultures are incubated for 3 to 7 days crude interferon on the order of 10.sup.3 units of interferon/ml. is produced.

Production of human IFN- .gamma. (immune) interferon

A process is disclosed for producing human immune interferon with cell cultures of human peripheral blood leukocytes modulated with Mezerein or 12, 13 phorbol dibutyrate and induced with the calcium ionophore A-23187. After cultures are incubated for 12 hours to about 7 days, crude interferon on the order of 10.sup.3 units of interferon/ml. is produced.

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Burke, VA, United States of America

Irwin A Braude