Applied Biosystems, LLC

Irudaya Charles

Alexander Vlassov

Susan Maylin Magdaleno

Nitin Puri

Christopher Burnett

Anilkumar R Kore

Shanmugasundaram Muthian

Chris Burnett

Susan Magdaleno

Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.

siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAI, and stabilized forms thereof

siRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof

RNAi sequence-independent modification formats, and stabilized forms thereof

SiRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof

The synthesis of capped/tagged RNA, methods of use and kits providing same are contemplated. Tagged RNA permits isolation of RNA transcripts in vitro. The ability to isolate and purify capped RNA results in improved transcription and translation and provides a tool for identifying RNA-protein interactions. Such capped RNA finds use in therapeutic applications, diagnosis and prognosis and in the treatment of cancers and HIV.

Preparation and isolation of 5' capped MRNA

Preparation and isolation of 5' capped mRNA

Growing community of inventors

Austin, TX, United States of America

Irudaya Charles