Enza Zaden Beheer B.v.

Stichting Dienst Landbouwkundig Onderzoek

Amphasys Ag

Iris Heidmann

Wouter Lindeman

Kimberly Boutilier

Grit Schade-Kampmann

Marco Di Berardino

Kim Kok

Brenda Johanna Maria De Lange

A method for the determination of pollen viability and/or maturation grade of a pollen population, comprising mechanically removing pollen grains from flowers; re-suspending pollen grains in an electrically conductive buffer for keeping the plant cells to remain viable; passing the pollen suspension through an appropriate filter with a pore size suitable for a microfluidic device being adapted to perform impedance flow cytometry (IFC); An advantage of the present invention is that a plant cell does not have to be stained for viability analysis, the method is non-invasive and a high number of cells can be analyzed in a short time frame in real-time and on-site. It is possible to follow developmental processes of plant cells, and that it is applicable to all plant species. In particular, the method allows a standardized measurement independent of the location and all over the world.

Method for determination of pollen viability and/or maturation grade of a pollen population

The present invention relates to a white-floweredplant, comprising a gene encoding the bacterial spot disease resistance protein 4 (Bs4), wherein the gene is derived from a purple-floweredspecies plant and the gene conveys resistance to a bacterial spot pathogen disease caused bypv.. The invention also relates to a white-floweredplant, comprising a gene encoding the bacterial spot disease resistance protein 4 (Bs4), wherein the plant is resistant to bacterial spot pathogen disease caused bypv.race I, II, III, IV, and VI. The invention further relates to a seed that produces a white-floweredplant, comprising a gene encoding Bs4, and pepper fruit produced by a white-floweredplant, comprising a gene encoding Bs4.

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A method for providing a transgenic plant, including transforming a plant cell or plant material with a nucleotide sequence coding for a BBM protein, wherein activity of the BBM protein is induced during transformation and/or regeneration of the transformed plant cell or plant material, and wherein the plant cell or plant material originates from a recalcitrant plant is provided. In addition, a transgenic plant or material thereof having a BBM protein, wherein the transgenic plant is a recalcitrant plant is also provided.

Method for providing fertile plants via induction of BBM during transformation

The present invention relates to a method for transferring one or more genetic traits from a purple-floweredspecies plant comprising said genetic trait, to a white-floweredspecies plant, said method comprising the steps of: (a) preparing a first hybrid plant comprising said genetic trait by crossing a first purple-floweredspecies comprising said genetic traits with a second purple-floweredspecies; (b) preparing a second hybrid plant by crossing said first hybrid plant with a first white-floweredspecies plant; (c) crossing said second hybrid plant with said first purple-floweredspecies plant to produce first progeny plants; and (d) preparing a third hybrid plant by crossing the first progeny plants obtained in step (c) with a second white-floweredspecies plant to produce second progeny plants, and selecting the third hybrid plant from the second progeny plants comprising said one or more genetic traits.

Method for transferring one or more genetic traits from a plant of the purple-flowered capsicum species to a plant of the white-flowered capsicum species

Growing community of inventors

Enkhuizen, Netherlands

Iris Heidmann