Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Innsu Daniel Kim

Hai Quang Tran

Alexandre Richez

Lewis J Kraft

Sean M Ramirez

Brian Douglas Mather

Xavier Von Hatten

Ludovic Vincent

Danny Yuan Chan

Hong Xie

Julia Morrison

Gianluca Andrea Artioli

Wei Wei

Edwin Li

Maxwell Zimmerley

Hayden Black

Krystal Sly

Avishek Aiyar

Sojeong Moon

Ryan Sanford

Michael Rapp

Jonathan Ziebarth

Casey Scott Irvin

An example flow cell includes a patterned substrate having an active region and a bonding region that at least partially surrounds the active region. The active region includes first depressions defined in a layer of the patterned substrate, surface chemistry positioned in the first depressions, and first interstitial regions surrounding the first depressions. The bonding region includes second depressions defined in the layer and second interstitial regions surrounding the second depressions. An adhesive is positioned over the second depressions and over the second interstitial regions. A cover is attached to the adhesive such that a flow channel is defined between a portion of the cover and the active region.

Flow cells with patterned bonding regions

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

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San Diego, CA, United States of America

Innsu Daniel Kim