Hoffmann-la Roche Inc.

Anaphore, Inc.

Ingrid Kjoller Larsen

Thor Las Holtet

Michael Etzerodt

Niels Jonas Heilskov Graversen

Jette Sandholm Kastrup

Bettina Bryde Nielsen

Hans Christian Thogersen

The present invention relates to the design of trimeric polypeptides using polypeptide structural elements derived from the tetranectin protein family, and their use in rational de novo design and production of multi-functional molecules including the application of the multi-functional molecules in protein library technology, such as phage display technology, diagnostic and therapeutic systems, such as human gene therapy and imaging. The trimeric polypeptides being constructed as a monomer polypeptide construct comprising at least one tetranectin trimerising structural element (TTSE) which is covalently linked to at least one heterologous moiety, said TTSE being capable of forming a stable complex with two other TTSEs; or as an oligomer which is comprised of two monomer polypeptide constructs as mentioned above, and which comprises three TTSEs or a multiplum of three TTSEs, or which is comprised of three monomer polypeptide constructs.

Trimerising module

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Charlottenlund, Denmark

Ingrid Kjoller Larsen