Arrowhead Madison, Inc.

Axolabs Gmbh

Noxxon Pharma Ag

Ingo Röhl

Jon Asher Wolff

Peter Mohr

David B Rozema

Darren H Wakefield

Philipp Hadwiger

David L Lewis

Torsten Hoffmann

Sven Klussmann

Eric Argirios Kitas

Andrei V Blokhin

Werner Purschke

Dirk Eulberg

Jeffrey Carlson

Jonathan D Benson

Axel Vater

Hans Martin Mueller

Kerstin Jahn-Hofmann

Günther Ott

Nadine Dörfler

Julia Knis

Provided herein is a method for detecting at least two distinct oligonucleotides of equal length in parallel from one biological sample, comprising the steps of providing a biological sample containing or suspected of containing oligonucleotides of interest; forming a hybridization mixture using at least two fluorescently labelled detection molecules with different surface charges; separating the detection molecules hybridized to the oligonucleotides by anion exchange HPLC; and detecting the hybridized detection molecule-oligonucleotide moieties by quantitative fluorescence readout. In a further aspect, a kit comprising at least two detection molecules is provided. In another aspect, provided herein is the use of at least two detection molecules with different surface charges for quantitatively detecting at least two distinct oligonucleotides of equal length in parallel from one biological sample.

Simultaneous detection of oligonucleotides, a kit and a use related thereto

The present invention is directed compositions for targeted delivery of RNA interference (RNAi) polynucleotides to hepatocytes in vivo. Targeted RNAi polynucleotides are administered together with co-targeted melittin delivery peptides. Delivery peptides provide membrane penetration function for movement of the RNAi polynucleotides from outside the cell to inside the cell. Reversible modification provides physiological responsiveness to the delivery peptides.

Peptide-based in vivo siRNA delivery system

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Memmelsdorf, Germany

Ingo Röhl