Avl Medical Instruments Ag

Presens Precision Sensing Gmbh

Other

Ingo Klimant

Otto S Wolfbeis

Marco J Leiner

Tobias Werner

Ute Kosch

Hellfried Karpf

Gerhard Holst

Luminescent microparticles and nanoparticles are provided for use either as internal standards for referencing fluorescent signals, or as markers for detecting biomolecules. Luminescence dyes are incorporated in inert form into solid materials such that they are protected from the influence of chemical and biological compounds in aqueous sample constituents. The photophysical characteristics of the dyes (spectral characteristics, luminescence quantum efficiency, luminescence fading time and polarization) remain unaffected. The surface of the nanoparticles and microparticles can be provided with reactive surfaces in order to enable covalent coupling of biochemicals or to eliminate aggregation.

Production and use of luminescent microparticles and nanoparticles

A method and device for fluorimetric determination of a biological, chemical or physical parameter of a sample utilize at least two different luminescent materials, the first of which is sensitive to the parameter, at least with respect to luminescence intensity, and the second of which is insensitive to the parameter, at least with respect to luminescence intensity and decay time. The luminescent materials have different decay times. The time- or phase behaviour of the resulting luminescence response is used to form a reference value for determination of a parameter.

Method and device for referencing fluorescence intensity signals

Optical temperature sensors and optical-chemical sensors with optical temperature compensation

The sensor membrane of an optical sensor for detection of O.sub.2, H.sub.2 O.sub.2, SO.sub.2 or halogenated hydrocarbons in a sample contains an indicator substance that is homogeneously immobilized in the polymer matrix of the sensor membrane and is, at least indirectly, in contact with the sample, changing at least one of its optical properties upon a change of the parameter to be measured. The indicator substance contains an inorganic salt of a transition metal complex with .alpha.-diimine ligands. The indicator substance is homogeneously distributed in the polymer matrix, which essentially consists of at least one substance belonging to the group of cellulose derivatives, polystyrenes, polytetrahydrofuranes, or their respective derivatives.

Sensor membrane of an optical sensor

In a sensor layer for quantitative determination of at least one chemical component in a gaseous or liquid sample medium containing a chromophore which is directly or indirectly responsive to the component being determined by changing its absorption spectrum, and a luminophore which is not responsive to the component being determined, where there is an at least partial overlap between the emission spectrum of the luminophore and the absorption spectrum of the chromophore, and where the energy transfer between luminophore and chromophore produces a measurable change in at least one luminescence characteristic of the luminophore, the luminophore L and the chromophore .GAMMA. are ionic substances with differing electrical charges, which are incorporated in a matrix material that is permeable to the chemical component being determined.

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Wuppertal, Germany

Ingo Klimant