Amoco Corporation

Indrani Mukharji

Friedhelm L Brinkhaus

James G Yarger

Rodney L Ausich

Huei-Che B Yen

John H Proffitt

Court A Saunders

DNA segments encoding the Erwinia herbicola enzymes geranylgeranyl pyrophosphate (GGPP) synthase, phytoene synthase, phytoene dehydrogenase-4H, lycopene cyclase, beta-carotene hydroxylase, and zeaxanthin glycosylase, DNA variants and analogs thereof encoding an enzyme exhibiting substantially the same biological activity, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes, zeaxanthin and zeaxanthin diglucoside by recombinant DNA technology in transformed host organisms are disclosed.

Biosynthesis of zeaxanthin and glycosylated zeaxanthin in genetically

DNA segments encoding the Erwinia herbicola enzymes geranylgeranyl pyrophosphate (GGPP) synthase, phytoene synthase, phytoene dehydrogenase-4H and lycopene cyclase, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes and beta-carotene by recombinant DNA technology in transformed host organisms are disclosed.

Beta-carotene biosynthesis in genetically engineered hosts

DNA segments encoding the Erwinia herbicola enzymes geranylgeranyl pyrophosphate (GGPP) synthase and phytoene synthase, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes and phytoene by recombinant DNA technology in transformed host organisms are disclosed.

Phytoene biosynthesis in genetically engineered hosts

DNA segments encoding the Erwinia enzymes geranylgeranyl pyrophosphate (GGPP) synthase, phytoene synthase and phytoene dehydrogenase-4H, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes and lycopene by recombinant DNA technology in transformed host organisms are disclosed.

Lycopene biosynthesis in genetically engineered hosts

A method of increasing the accumulation of squalene and specific sterols in yeast comprising increasing the expression level of a structural gene encoding a polypeptide having HMG-CoA reductase activity in a mutant yeast having single or double defects in the expression of sterol biosynthetic enzymes is provided. The expression level of a structural gene is preferably increased by transforming yeast with a recombinant DNA molecule comprising a vector operatively linked to an exogenous DNA segment that encodes a polypeptide having HMG-CoA reductase activity and a promoter that is suitable for driving the expression of the encoded polypeptide in the transformed yeast. The polypeptide having HMG-CoA reductase activity is preferably a truncated, active HMG-CoA reductase enzyme. Recombinant DNA molecules useful for transforming yeast and mutant yeast transformed with such recombinant DNA molecules are also disclosed.

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Evanston, IL, United States of America

Indrani Mukharji