Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Danstar Ferment Ag

Universiteit Stellenbosch

Indraneel Shikhare

Emily Stonehouse

Haowen Xu

Aaron Argyros

Trisha Barrett

Nicky Caiazza

Vineet Rajgarhia

Merja Penttilä

John E McBride

Erin Wiswall

Charles F Rice

Allan C Froehlich

Anne K Warner

Mark Mellon

Ryan Skinner

Riaan Den Haan

Shital A Tripathi

Chhayal V Gandhi

Kristen M Deleault

John S Bardsley

Heidi H Hau

Frank Agbogbo

Kevin S Wenger

Jennifer Gosselin

Yin-Ying Chiu

Vineet B Rajgarhia

Alan Belcher

Abigail Foster

Elena E Brevnova

Alan Benjamin Gilbert

Deidre Willes

Jim Flatt

Matti Siika-Aho

Marja Ilmen

Emile Van Zyl

Danie Lagrange

Elena Brevnova

Naomi Thorngren

Heidi Lau

Merja Penttila

Abigail S Foster

Chhayal Ghandi

Jeffrey D Keating

Emily A Stonehouse

Heidi Hanson Hau

Dan Murphy

Alan Froehlich

Jeff Villari

John E Mcbride

Jaana Uusitalo

Shaunita Rose

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

Expression of enzymes in yeast for lignocellulose derived oligomer CBP

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Lebanon, NH, United States of America

Indraneel Shikhare