Council of Scientific and Industrial Research

Indra Sandal

Amit A Bhattacharya

Paramvir Singh Ahuja

Ashu Gulati

Ajay Kumar

Madhu Sharma

Ravindranath Srigiripuram Desikalhar

P S Ahuja

Srigiripuram D Ravindranath

The present invention relates to a thermolabile caffeine fraction useful for an efficient-mediated genetic transformation in plant systems to develop desired traits in plant, and a method of preparing said fraction from tea leaves and also, an efficient and cost-effective method of introducing said-mediated genetic transformation into plant systems using said caffeine fraction of tea leaves.

[object Object]

The present invention relates to a thermolabile caffeine fraction useful for an efficient-mediated genetic transformation in plant systems to develop desired traits in plants, and a method of preparing said fraction from tea leaves and also, an efficient and cost-effective method of introducing said-mediated genetic transformation into plant systems using said caffeine fraction of tea leaves.

The present invention relates to an efficient and cost effective method of preventing growth of genetic transformant bacteriaafter transformation in plants by using tea leaf extract as a bactericide, wherein said method leads to elimination of common problem of polyphenol oxidation during transformation and thereby helps maintain regeneration potential in explants and also helps in increased transformation efficacy

Efficient method of preventing growth of microbial genetic transformant after transformation

The present invention relates to Production of transgenic tea ((L.) O. Kuntze) through biolistic.

Transgenic tea through biolistic using leaf explants

The present invention relates to novel method for micropropagation of tea plants from explants obtained from completely folded, half opened or fully expanded leaves via callus phase, by culturing the explants on different media.

Method for microproduction of tea plants from leaf explants

The invention provides a one step method for micropropagation of tea plants from leaf explants via callus phase, wherein the explants are maintained in basal Murashige and Skoog medium (Murashige T. and Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497; 1962) supplemented with vitamins like thiamine-HCl (0.05 to 2.0 mg/l), pyridoxine-HCl (0.25 to 1.5 mg/l) and nicotinic acid (0.25 to 1.5 mg/l) together with glycine (1.0 to 3.0 mg/l) and either 2.5, 5.0 or 7.5 mg/l 2,4-Dichlorophenoxy acetic acid for a period of 14-16 weeks, from the stage of induction of callus to rhizogenesis and shoot formation.

Growing community of inventors

Himachal Pradesh, India

Indra Sandal