10x Genomics, Inc.

Theranos, Inc.

Labrador Diagnostics LLC

Indira Wu

Pranav Patel

Benjamin J Hindson

Keith P Bjornson

Paul Hardenbol

Kamila Belhocine

Paul William Wyatt

Phillip Belgrader

Serge Saxonov

Rajiv Bharadwaj

Christopher Hindson

John R Stuelpnagel

Katherine Pfeiffer

Andrew D Price

Michael Ybarra Lucero

Scott M Tabakman

Geoffrey McDermott

Tarjei Sigurd Mikkelsen

Tobias Daniel Wheeler

Zachary Bent

Aaron Richardson

Paul Ryvkin

Zahra Kamila Belhocine

Josephine Lee

Jessica Michele Terry

Mohammad Rahimi Lenji

Josephine Harada

Vijay Kumar Sreenivasa Gopalan

Elliott Meer

Solongo B Ziraldo

Niranjan Srinivas

Stephane Claude Boutet

Sarah Taylor

Christopher Joachim O'Keeffe

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same

Improved methods for amplifying target nucleic acid sequences are provided by 1) first amplifying the number of copies of target nucleic acid sequences in a sample by a first nucleic acid amplification method, and then 2) applying a second nucleic amplification method to the amplified sample, or aliquot thereof, further amplifying the number of copies of target sequences. In embodiments, a first nucleic acid amplification method is a thermocycling method, and a second nucleic acid amplification method is an isothermal method.

Hybrid multi-step nucleic acid amplification

Methods and compositions for the identification of genetic-related information are provided. At least portions of methods provided herein may be performed without thermocycling. Methods and compositions may include reagents such as nucleic acid polymerases and primers.

Diagnostic methods and compositions

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San Carlos, CA, United States of America

Indira Wu