Korea Research Institute of Bioscience and Biotechnology

Korea Research Institute of Bioscience

Seoul National University

Snu Rdb Foundation

In Pyo Choi

Suk Ran Yoon

Ki-Hyun Kim

Junho Chung

Yee Sook Cho

Hyori Kim

Jung Weon Lee

Su Ui Lee

Binna Seol

Sang Jick Kim

Se Mi Kim

Sooyun Lee

Han-Seop Kim

Soo Yeon Park

Han Na Kim

Sol Ji Jung

Tae-Don Kim

Hye Jin Min

Jin Myeong Song

Ji Hye Ryu

The present disclosure relates to a method for producing of a memory-like natural killer cell having an ability to produce a higher level of a natural killer cell receptor, having a better killing capacity, and having an ability to produce a higher level of IFN-γ than a natural killer cell in human peripheral blood, and a memory-like natural killer cell produced by the method, and a cancer treatment method using the memory-like natural killer cell.

Method for producing memory-like NK cells with ability to express higher levels of NCRs, cytotoxicity, and IFN-γ than NK cells in human peripheral blood

The present invention relates to a method for producing natural killer cells using direct reprogramming, natural killer cells produced thereby, a biomarker specific to the natural killer cells, a cell therapeutic agent comprising the natural killer cells, a composition for treatment and prevention of cancer, a cryopreservation cell vial for storing the natural killer cells, and a medium kit for inducing the direct reprogramming. Exhibiting excellent proliferative potential and cancer cell killing potential, the natural killer cells produced by the production method can be effectively utilized for mass production and in a composition for treatment and prevention of cancer.

Method for producing natural killer cell and use thereof

Disclosed is a method for producing a large amount of natural killer cells and the use of the natural killer cells as an anticancer agent. The method produces fresh NK cells with high purity within a short time, and can also produce cold-preserved NK cells and thawed cryopreserved NK cells having efficacy comparable to the fresh NK cells. NK cells having efficacy comparable to the fresh NK cells can also be produced from cryopreserved CD3-negative cells. The fresh NK cells, cold-preserved NK cells and cryopreserved NK cells exhibit therapeutic effects against various cancers, including colorectal cancer, lung cancer, liver cancer, pancreatic cancer and leukemia, indicating these NK cells are effective as cellular therapeutic agents. Also disclosed are doses and methods of administration that show excellent effects when the fresh NK cells, cold-preserved NK cells and cryopreserved NK cells are used as pharmaceutical compositions for cellular therapy.

Method for mass producing natural killer cell and use of natural killer cell obtained by the method as anti-cancer agent

Disclosed are a natural killer (NK) cell expressing an anti-cotinine chimeric antigen receptor (CAR) specifically binding to cotinine, and a cell therapeutic agent containing the NK cell. The CAR-expressing NK cell which specifically binds cotinine, can effectively move to tumor tissue, regardless of the kind of cancer, depending on the binding substance bound to cotinine. Therefore, the natural killer cell can be usefully employed as a gene therapy exhibiting a highly efficient anticancer effect.

Natural killer cell expressing anti-cotinine chimeric antigen receptor

Growing community of inventors

Daejeon, South Korea

In Pyo Choi