California Institute of Technology

Imran R Malik

Axel Scherer

Xiomara Linnette Madero

Erika F Garcia

Sheel Mukesh Shah

Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.

Multiplexing and quantification in PCR with reduced hardware and requirements

Devices and system for preparing samples are described. Such devices can comprise fluidic chambers, reservoirs, and movable structures for controlling the movement of samples. The device can also comprise functional elements for performing specific operations.

Sample preparation devices and systems

Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers. The device may have a reaction cartridge with a reaction chamber to perform techniques such as polymerase chain reaction.

Devices and methods for biological sample-to-answer and analysis

Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

Instruments for biological sample-to-answer devices

Instruments for biological sample preparation devices

A method of fabricating a fluidic device comprises providing a fluidic device including a body having a surface and one or more channels located in the body. Recesses are defined on said surface. The one or more channels can have respective boundaries. A layer of adhesive including one or more panel-shaped pieces having a pattern based on the pattern of boundaries of the channels can be formed and applied on the surface of the body. It is further controlled that the layer of adhesive has respective boundaries surrounding the boundaries of the one or more channels.

Fluidic devices and fabrication methods for microfluidics

Devices and methods for biological sample preparation

Methods and devices for biological sample preparation and analysis are disclosed. A device may have a circular arrangement of containers, with a connecting structure such as a disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. The device may be pen-shaped and have a sample drawing needle connected to the containers.

Pen-shaped device for biological sample preparation and analysis

Temperature control devices and methods are described. The described temperature control devices and methods comprise optical emission and detection assemblies and can be used in PCR and qPCR applications.

Temperature control devices and methods

Methods for measuring and analyzing biological or chemical samples using consumer electronic devices and systems are described. Moreover, an accessory to enable using devices such as cell phones and smartphones with fluidic illumination chambers are described.

Methods for measuring samples using consumer electronic devices and systems

Methods to fabricate reaction cartridges for biological sample preparation and analysis are disclosed. A cartridge may have a reaction chamber and openings to allow fluids to enter the chamber. The cartridge may also have handles to facilitate its use. Such cartridges may be used for polymerase chain reaction.

Methods of fabrication of cartridges for biological analysis

Structures and methods are described for optical detection of physical, chemical and/or biological samples. An optical detection structure may include a LED source, multiple filters and single or multiple sample areas. A detector may be used to record a fluorescence signal. The sample area may allow the introduction of removable cartridges.

Optical technique for chemical and biochemical analysis

Optical devices and methods for measuring samples while minimizing stray light are described. Such methods and devices are applicable to multiple fluid chambers with multiple sources as an integrated optical element. Light sources can be embedded onto a chip or microarray with multiple chambers, or can be part of an instrument arrangement.

Growing community of inventors

Pasadena, CA, United States of America

Imran R Malik