Egyt Gyogyszervegyeszeti Gyar

Medimpex Gyogyszerkulkereskedelmi Vallalat

Ilona Beladi

Miklos Toth

Helga Hammer

Valeria Endresz

Sandor Toth

Ilona Kahan Nee Laszlo

The invention relates to a process for the preparation of .alpha.- and .gamma.-interferon by isolating the buffy coat fraction of blood, removing the erythrocytes, suspending the leukocytes in a suitable nutrient medium and treating with an .alpha.-interferon inducer and with a .gamma.-interferon inducer, which comprises producing .alpha.-interferon and .gamma.-interferon one after the other in the same leukocyte culture by pre-treating the suspension obtained after the separation of the leukocyte/buffy coat/fraction of blood, removal of the erythrocytes and suspending the leukocytes in a suitable nutrient medium with - or .beta.-interferon, contacting with an .alpha.-interferon inducer, separating the liquid containing the .alpha.-interferon from the cells, if desired recovering the -interferon, washing and suspending the cells in a suitable nutrient medium, treating with a mitogenic agent, separating the liquid comprising .alpha.-interferon from the cells and if desired recovering .alpha.-interferon and if desired making .alpha.-interferon free of .alpha.-interferon. The advantage of the process is that .alpha.- and .gamma.-interferon are produced from the same leukocyte culture in two steps. This makes the process more economical and enables an effective utilization of the limitedly available leukocytes.

Process for the preparation of human leukocyte and human gamma interferon

Tri-tetracyclines are complexes of tetracyclines with an amino compound containing per molecule at least a tris (hydroxyalkyl) group and at least a primary or secondary amino group in a molar excess in respect to the tetracycline compound. The complexes have high water solubility and are particularly useful for parenteral administration.

Therapeutically active complexes of tetracyclines

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Szeged, Hungary

Ilona Beladi