Wallac Oy

Pharmacia Ab

Lkb-Produkter Ab

Ilkka Hemmila

Veli-Matti Mukkala

Timo Lovgren

Marek Kwiatkowski

Erkki J Soini

Harri Hakala

Harri Takalo

Jan P Carlsson

Christian Sund

Pertti Hurskainen

Jouko Kankare

Kaj Blomberg

Hakan Drevin

Sven O Oscarsson

Heikki Mikola

Martti Latva

Salifu Dakubu

Jyrki Ylikoski

Anna T Martin

Bifuncitonal chelating pyridine compound and its use for conferring chelating properties on organic compounds. The pyridine compound has the structure ##STR1## where (i) n is an integer 1 or 2, (ii) R.sub.1, R.sub.2 and R.sub.3 represent groups that have no electrons capable of significantly delocalizing or resonating with the pyridine ring, such as hydrogen, alkyl or aralkyl having an aliphatic carbon atom next to the pyridine ring; at least two of R.sub.1, R.sub.2 and R.sub.3 being hydrogen, iii) Z an Z' represent identical or different structures, each of which comprises at least one heteroatom having a free pair of electrons as that the said at least one heteroatom together with the nitrogen atom of the pyridine ring is capable of chelating a metal ion, iv) - - - - specifies that the group X-Y is a substituent replacing a hydrogen anywhere in the parent pyridine compound, and v) X-Y represents an organic group which is inert to said chelation, and in which iX is an inert and stable bridge and Y is a functional group or a residue of an organic compounbd that has properties conferred on the compound of formula II; said group X-Y being linked to the pyridine ring of formula II via an aliphatic carbon atom attached to and acid ester, salt and chelate forms thereof involving at least one of said chelating heteroatoms.

Metal-chelating 2,6-disubstituted pyridine compounds and their use

The invention is a luminescence energy transfer based bioaffinity assay comprising a first group labeled with an energy donating compound (donor) and a second group labeled with an energy accepting compound (acceptor), wherein the donor is a luminescent lanthanide chelate having a long excited state lifetime and the acceptor is either a luminescent compound having a short excited state lifetime or a nonluminescent compound. The increase or decrease, respectively, in the energy transfer from the donor to the acceptor resulting from shortening or lengthening, respectively, of the distance between the said groups, is measured. Characteristic for the invention is that the lanthanide energy emission and the acceptor energy absorption do not essentially or not at all overlap each other.

Homogeneous luminescence assay method based on energy transfer

Method for a homogeneous biospecific affinity assay for determining the content of a substance (analyte) in a biological sample. The assay is carried out in an aqueous reaction medium by means of time-resolved fluorescence spectroscopy and with a biospecific affinity reactant labeled with a lanthanide chelate in which a lanthanide ion exhibiting ionic fluorescence is chelated by a ligand bound covalently to the reactant. The characteristic feature is

Modulated homogeneous fluorescence biospecific affinity assay employing

A method of conducting assays for analytes, usually from biological samples, utilizing bioaffinity reagents linked to luminescent lanthanide chelates, which are synthesized so that the non-radiative quenching of the ion luminescence through C--H bond vibrational energy manifolds is avoided using a stable chelate where the CH and CH.sub.2 groups in the vicinity of the emittive ion are substituted with CD and CD.sub.2 groups hence producing improved luminescence quantum yield and higher assay sensitivities.

Luminescent lanthanide chelates with decreased non-radiative energy loss

Enzyme activity determinations method characterized by the using of

Compound having the following structure: ##STR1## R is a direct chain or branched alkylene group comprising 2-8 carbon atoms,

Determination method utilizing reagents covalently labelled with

Method for quantitative determination of a biospecific affinity reaction in which an immunochemical compound labelled with a lanthanide chelate is used which can be detected by means of time-resolved fluorescence in which the amount of labelled compound present in the biospecific affinity reaction can be measured without the separation of free and biospecifically bound labelled compound having to be carried out.

Method for quantitative determination of a biospecific affinity reaction

Method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lanthanide chelate complex formed by a lanthanide coupled to the substance via a chelate forming compound, such as an EDTA-analogue, the lanthanide ion before the detection is dissociated from the active substance for instance by adding a buffer with a pH value below 3.5 and a splitting detergent, whereafter the excitation in the determination takes place in the solution in the presence of a .beta.-diketone by using a short radiation pulse and the fluorescence from the marker is detected when the fluorescence from the noise sources has substantially ceased.

Method for fluorescence spectroscopic determination of a biologically

An improved method of determining the nature of a substance by fluoroscence spectroscopy wherein a fluorescent marker is coupled to the molecules of the substance comprises the use of a marker having a longer period of fluorescence than those of possible sources of noise and by employing an exciting radiation pulse of short duration so that the fluorescence of the marker is detected after the objectionable sources of fluorescence have ceased; the marker including a fluorescent lanthanide chelate complex.

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Turku, Finland

Ilkka Hemmila