Director-general of Agency of Industrial Science and Technology

National Institute of Advanced Industrial Science and Technology

Agency of Industrial Science and Technology

Ikuo Matsui

Kazuhiko Ishikawa

Yoshitsugu Kosugi

Koichi Honda

Hiroyasu Ishida

Sachio Miyairi

Eriko Matsui

Satoko Kawasaki

Katsuhiko Higuchi

Yuji Urushibata

Hiroki Higashibata

Yasutaka Tahara

Joh Yong-Goe

Yutaka Kawarabayashi

A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from, a hyperthermophilic archaeon.

DNA replication factors

A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.

Flap endonuclease mutants

The invention relates to a thermophilic enzyme having &#x3b2;-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.

Methods for making and using a thermophilic enzyme as a &#x3b2;-glycosidase

The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such asin the usual manner to create a transformant, and culturing the transformant.

DNA helicase

A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90&deg; C. or higher, and the transformed host cell is cultured.

Thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity

The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75&deg; C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75&deg; C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.

Thermostable flap endonuclease derived from hyperthermophile bacterium belonging to the genus pyrococcus

Thermostable flap endonuclease derived from a hyperthermophile bacterium belonging to the genus pyrococcus

An acyl peptide hydrolase having an optimum temperature range of 90-95.degree. C. and a gene encoding the same are disclosed. With the above enzyme, it becomes possible to conduct amino terminal analysis of acylated proteins and peptides at high temperatures.

Thermostable acyl peptide hydrolase and gene encoding the same

Disclosed are a thermophilic phospholipase having an optimum temperature range of 95 to 105.degree. C. which is useful in high-temperature degumming processes in oil refining process and high-temperature processing of phospholipids; and a method for producing a thermophilic phospholipase comprising culturing a microorganism capable of producing the phospholipase in a culture medium and collecting the phospholipase from the resultant culture.

Thermophilic phospholipases and method for production thereof

There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.

Growing community of inventors

Ibaraki, Japan

Ikuo Matsui