Takara Shuzo Co., Inc.

Takara Bio Inc.

Other

Mie University

Nippon Kayaku Kabushiki Kaisha

The Johns Hopkins University

Rutgers, the State University of New Jersey

Osaka University

Horiba, Ltd.

Fujita Health University

National Food Research Institute

Research Institute for Glycotechnology

Irica Instruments, Inc.

Takaru Shuzo Co., Ltd.

Ikunoshin Kato

Hiroaki Sagawa

Kiyozo Asada

Nobuto Koyama

Katsushige Ikai

Kazutoh Takesako

Takashi Uemori

Takeshi Sakai

Tatsuji Enoki

Hiroyuki Mukai

Jun Tomono

Eiji Nishiyama

Yoshimi Sato

Takanari Tominaga

Junichi Mineno

Hikaru Takakura

Kimikazu Hashino

Fusao Kimizuka

Takashi Okado

Kaoru Kojima

Hiromu Ohnogi

Naoyuki Awazu

Mutsumi Sano

Yoshiko Nomura

Masanori Mitta

Kazue Miyake

Takashi Ueno

Hideto Chono

Kazuo Shimanaka

Mio Morishita

Akihiro Kondo

Masanori Takayama

Hitomi Kimura

Toru Kurome

Hua-Kang Wu

Harumi Ueno

Hiroshi Shiku

Yoshizumi Ishino

Mitsuko Ideno

Mitsuhiro Ueno

Susumu Tsunasawa

Hiroyuki Izu

Michio Hagiya

Yoshikuni Nakanishi

Fu-Gong Yu

Nobuko Muraki

Yoh'ichi Ohdate

Tomoko Fujita

Osamu Takeda

Shigekazu Hokazono

Takashi Kawai

Masamitsu Shimada

Fumitsugu Hino

Makoto Ito

Yoshihisa Umeda

Hirokazu Kotani

Hideyo Yamaguchi

Katsuhiko Yamamoto

Tomoko Shimojo

Hiroaki Ikeda

Yoshie Yoshikawa

Shigetoshi Mizutani

Kazuyori Ochiai

Tsuyoshi Miyamura

Yoshimi Onishi

Masahiro Endo

Kaoru Inami

Takahiro Marui

Kazue Nishiwaki

Akihiko Kita

Tetsuo Shiba

Junko Yamamoto

Masanari Kitagawa

Hirofumi Yoshioka

Atsushi Oshima

Sumiko Akiyoshi

Toshiharu Ohba

Makoto Moriguchi

Kazuhiko Nishitani

Kaoru Katayama

Masayori Inouye

Tetsushi Saino

Akira Obayashi

Nozomu Okino

Kei Fujinaga

Sachiko Okamoto

Fuyuko Takashima

Kenji Sobue

Eiji Kobayashi

Ichiro Azuma

Naoyuki Taniguchi

Yoshikazu Kurosawa

Susumu Matsui

Hiromi Ohkawa

Toshikazu Nishie

Yuan C Lee

Keiji Tanaka

Takeshi Mori

Yoshinobu Okuno

Christian B Anfinsen

A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.

Composition for detection of RNA

A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.

Nucleic acid for treatment or prevention of immunodeficiency virus infection

Disclosed is a method for enhancing the function of a T cell, which is characterized by inhibiting the expression of programmed death-1 ligand 1 (PD-L1) and/or programmed death-1 ligand 2 (PD-L2) in the T cell. Also disclosed is a function-enhanced T cell which is produced by the function enhancement method. Further disclosed is a therapeutic agent comprising the function-enhanced T cell. The T cell can enhance an immune response to cancer, and is useful in an immunotherapy effective for cancer and the treatment or prevention of infectious diseases and autoimmune diseases.

Method for enhancing a function of a T cell

The present invention provides cold shock protein-containing compositions for improved DNA synthesis reactions with improved reactivity, methods for synthesizing DNA using such compositions, kits for use in such methods, and DNA compositions yielded by such methods. The present invention further provides cold shock protein-containing compositions for the identification of endoribonuclease cleavage sites, methods for identifying endoribonuclease cleavage sites using such compositions, and kits for use in such methods.

Cold shock protein compositions and methods and kits for the use thereof

Disclosed is a cell which can express a non-natural oligomeric protein, which has, introduced therein, a gene encoding an exogenous polypeptide corresponding to at least one endogenous polypeptide constituting a natural oligomeric protein, and in which the expression of the endogenous polypeptide is inhibited.

Method for expression of specific gene

The present invention relates to a method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors under nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle. The present invention allows induction and growth of pluripotent stem cells at high frequency, and it also allows production of pluripotent stem cells with high efficiency. The nuclear reprogramming factors to be used may be any selected from the group consisting of OCT4, SOX2, c-MYC, KLF4, NANOG and LIN28.

Method for production of pluripotent stem cell

Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject.

Cell capable of expressing exogenous GITR ligand

The N-acetylglucosaminyltransferase III activity is enhanced in a cell carrying retrovirus-origin gag-pol gene and env gene. By constructing a retrovirus vector with the use of the above cell, a retrovirus vector having a modified sugar chain structure can be obtained. The retrovirus vector constructed by this method shows a high infection efficiency particularly in the presence of a functional substance.

Method for producing retrovirus using human producer cells with increased N-acetylglucosaminyltransferase III activity

The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof.

Process for producing cytotoxic lymphocyte

The present invention provides a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one step selected from induction, maintenance and expansion of a cytotoxic lymphocyte using a medium containing serum and plasma at a total concentration of 0% by volume or more and less than 5% by volume, in the presence of fibronectin, a fragment thereof or a mixture thereof.

Process for producing cytotoxic lymphocytes

A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.

Method of retrovirus storage

A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.

Method for transfer of gene into fat cell or progenitor fat cell

Growing community of inventors

Otsu, Japan

Ikunoshin Kato