Ajinomoto Co., Ltd.

Kyoto University

Ikue Harata

Sho Senda

Satoru Okamoto

Manabu Kitazawa

Yoko Kuriyama

Nao Sugimoto

Satoru Ohashi

Tomomi Yoshida

Tsuyoshi Kobayashi

Makoto Ikeya

Junya Toguchida

Yonghui Jin

Hiroki Ozawa

Mesenchymal stem cells may be culture for a long period, without using any special apparatus, equipment and the like, in a medium in which seven kinds of nonessential amino acids of glycine, alanine, serine, proline, asparagine, aspartic acid, and glutamic acid are reduced.

Culture medium for mesenchymal stem cells

Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a pharmaceutically acceptable salt thereof, and which is substantially free of &#x3b2;-mercaptoethanol or contains &#x3b2;-mercaptoethanol at a concentration of not more than 9 &#x3bc;M, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state.

Culture method for stable proliferation of pluripotent stem cell while maintaining undifferentiated state

A medium which comprises a fibroblast growth factor (FGF), and a sulfated compound or a pharmaceutically acceptable salt thereof at a concentration which promotes the growth of a stem cell in the presence of FGF, is useful for culturing stem cells.

Culture medium for proliferating stem cell, which contains sulfated compound

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Kawasaki, Japan

Ikue Harata