Wisconsin Alumni Research Foundation

Illumina Corporation

Illumina Cambridge Limited

Igor Yu Goryshin

William S Reznikoff

Kevin L Gunderson

Frank J Steemers

Natasha Pignatelli

Ramesh Vaidyanathan

Nicholas Caruccio

Mark A Maffitt

Thomas Royce

Bradley Baas

Hong Zhou

Sasan Amini

Molly Min He

Niall Anthony Gormley

Jerome J Jendrisak

Amirali Kia

Fiona Kaper

Todd A Naumann

Jason Richard Betley

Rigo Pantoja

Dmitry K Pokholok

Natalie Morrell

Fan Zhang

Wouter Meuleman

Rosamond Jackson

Avgousta Ioannou

Gareth Jenkins

Dona L York

Jacqueline Weir

Steven J Norberg

Casey Turk

Reza Kahlor

Reza Kalhor

Artificial transposon sequences having code tags and target nucleic acids containing such sequences. Methods for making artificial transposons and for using their properties to analyze target nucleic acids.

Linking sequence reads using paired code tags

Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

Contiguity preserving transposition

Embodiments of the present invention relate to sequencing nucleic acids. In particular, embodiments of the methods and compositions provided herein relate to preparing nucleic acid templates and obtaining sequence data therefrom.

Methods and compositions for nucleic acid sequencing

Transposomes and oligonucleotide replacement methods to make DNA libraries that have distinct 5' and 3&#x2032; tags, and to make directional libraries that are enriched for a desired strand.

Oligonucleotide replacement for di-tagged and directional libraries

Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.

Double transposition methods for manipulating nucleic acids

A system for in vitro transposition includes a donor DNA that includes a transposable element flanked by a pair of bacterial transposon Tn5 outside end repeat sequences, a target DNA into which the transposable element can transpose, and a modified Tn5 transposase having higher binding avidity to the outside end repeat sequences and being less likely to assume an inactive multimer form than wild type Tn5 transposase.

System for in vitro transposition

A method for making insertional mutations at random or quasi-random locations in the chromosomal or extra-chromosomal nucleic acid of a target cell includes the step of combining, in the target cell, cellular nucleic acid with a synaptic complex that comprises (a) a transposase protein and (b) a polynucleotide that comprises (1) a pair of nucleotide sequences adapted for operably interacting with Tn5 transposase and (2) a transposable nucleotide sequence therebetween, under conditions that mediate transpositions into the cellular DNA. In the method, the synaptic complex is formed in vitro under conditions that disfavor or prevent the synaptic complexes from undergoing productive transposition.

Method for making insertional mutations using a Tn5 synaptic complex

A method for making insertional mutations at random or quasi-random locations in the chromosomal or extra-chromosomal nucleic acid of a target cell includes the step of combining, in the target cell, cellular nucleic acid with a synaptic complex that comprises (a) a Tn5 transposase protein and (b) a polynucleotide that comprises a pair of nucleotide sequences adapted for operably interacting with Tn5 transposase and a transposable nucleotide sequence therebetween, under conditions that mediate transpositions into the cellular DNA. In the method, the synaptic complex is formed in vitro under conditions that disfavor or prevent the synaptic complexes from undergoing productive transposition.

Growing community of inventors

Madison, WI, United States of America

Igor Yu Goryshin