Roche Molecular Systems, Inc.

Illumina Corporation

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Igor Kozlov

Amar Gupta

Randall K Saiki

Alison Tsan

Randall Saiki

Dieter Heindl

Stephen Gordon Will

Chanfeng Zhao

Hannes Kuchelmeister

Galina Steinberg-Tatman

Cherie Holcomb

Marintha Heil

Alexander Nierth

Jeffery Fong

Concordio Anacleto

Christian Wellner

Ed Gustavo Marins

Aaron T Hamilton

Alan Casilla

Elizabeth Marie Scott

Ling Wang

Karan Kampani

Trevor Hryce

Nikolaus-Peter Stengele

Volker Gatterdam

Priya Bellare

Aaron Thaddeus Hamilton

Qiao Ruan

Methods for the rapid detection of the presence or absence of Hepatitis B Virus (HBV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, competitive blocking oligonucleotides, and probes targeting HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA) and kits are provided that are designed for the detection of HBV (in particular HBV RNA, in particular, HBV RNA transcribed from cccDNA, such as pgRNA).

Compositions and methods for amplification and detection of hepatitis B virus RNA, including HBV RNA transcribed from cccDNA

The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.

Methods for performing multiplexed real-time PCR

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

Photocleavable nucleotide reagents with high stability

The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.

Methods for performing multiplexed PCR

The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using TAGS hydrolysis probes.

Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch

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Danville, CA, United States of America

Igor Kozlov