Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Igor Cunha Oliveira

Rebecca E Cahoon

Bo Shen

Omolayo O Famodu

Mitchell Christian Tarczynski

Leslie T Harvell

William B Allen

Changjiang Li

Stephen M Allen

Anthony John Kinney

Keith S Lowe

Timothy George Helentjaris

Hajime Sakai

Dwight T Tomes

Theodore Mitchell Klein

Haiyin Wang

Xiaomu Niu

Deping Xu

Sabine U Epelbaum

Todd J Jones

Jijun Zou

Yinghong Li

Methods are provided for transforming isolated, immature maize embryos and for producing transgenic maize plants. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.

Method for obtaining transformed maize plants

The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.

Methods for large scale functional evaluation of nucleotide sequences in plants

The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Recombinant DNA construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.

Alteration of oil traits in plants

The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.

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Urbandale, IA, United States of America

Igor Cunha Oliveira