Eastman-kodak Company

Johnson & Johnson Clinical Diagnostics, Inc.

Other

Clinical Diagnostic Systems, Inc.

Indicon Inc.

Ignazio S Ponticello

Richard C Sutton

Susan J Danielson

Marsha D Oenick

Kenneth R Hollister

Richard C Tuites

Barbara A Brummond

John Bruce Findlay

David A Hilborn

Hans W Osterhoudt

Tobias E Ekeze

Kenneth G Christy, Jr

Mohan S Saini

Jerome C Swartz

Dennis R Zander

Philip S Bryan

James Albert Reczek

Hugh G McGuckin

Harold C Warren

Thomas J Cummins

Ronda E Factor

Fred T Oakes

Robert C Daly

Laurel J Pace

Michael J Hanrahan

William Harold Donish

David B LaTart

George Villard

Robert A Snow

Stephen C Hasselberg

Edward P Otocka

Sandra K Fischer

Wayne A Bowman

Tsang J Chen

John M Noonan

Glenn T Pearce

Thomas L Penner

Jon N Eikenberry

Michael R Roberts

David P Brust

Daniel S Daniel

Zona R Pierce

JoAnne H Kerschner

David S Frank

Richard L Detwiler

Thomas C Arter

Hyman L Cohen

John W Backus

Frederick R Koeng

Calvin Roman Messing

John M McIntire

Henry J Sniadoch

Elizabeth V Patton

David M Taylor

Marjorie M Datskow, Executrix

Susan C Gross, Deceased

Patricia N Tyminski

Paul Hong-Dze Chen

Nucleic acids can be amplified and detected using an element which has a sealable support on which is disposed a nucleic acid reagent composition. The composition is a mixture of a nucleic acid reagent composed of polymeric particles to which an oligonucleotide is covalently attached. The particles are prepared from a first polymer having a glass transition temperature of at least about 70.degree. C. and have an average diameter of from about 0.1 to about 3 micrometers. The reagent is adhered to the support using a water insoluble adhesive comprising a second polymer which has a glass transition temperature which is at least about 30.degree. C. less than the glass transition temperature of the first polymer. The adhesive is present in the composition at from about 1 to about 20 dry weight percent. The method provides high sensitivity and low background in the assay of nucleic acids, preferably using polymerase chain reaction.

Method for nucleic acid amplification and detection using adhered probes

A dry immunoassay analytical element, for assaying a ligand, comprising in the following order:

Immunoassay elements comprising polymers containing vandium IV (V+4) ions

The invention is directed to labeled drug hapten analogues comprising:

Immunoassay with novel labeled drug hapten analogues

Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.

Methods for capture and selective release of nucleic acids using weakly

An immunoassay method and analytical elements for detecting carbamazepine drugs, for example, in body fluids, is described. The immunoassay method comprises:

Immunoassays with novel labeled carbamazepine hapten analogues

A labeled carbamazepine analogue is described comprising:

Labeled carbamazepine hapten analogues for competitive enzyme

The invention is directed to an immunoassay for a thyronine derivative comprising:

Immunoassays with labeled thyronine hapten analogues

Weakly basic ethylenically unsaturated polymerizable monomers and polymers have been prepared. The monomers are represented by the structure (I): ##STR1## wherein R is hydrogen or methyl, and R.sup.1 is alkylene of 1 to 3 carbon atoms. The resulting polymers are water-soluble and cationic at acidic pH, but water-insoluble and neutral in charge at basic pH. The polymers are useful for precipitating nucleic acids.

Weakly basic polymerizable monomers and polymers prepared therefrom

A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydrogenase and an oxidized nicotinamide coenzyme. The element includes at least one reagent layer beneath a porous spreading layer. This reagent layer has a crosslinked polymeric binder prepared, in part, with a polymerizable monomer having a halomethylcarbonyl, haloethylcarbonyl, halomethylsulfonyl or haloethylsulfonyl group. Alcohol dehydrogenase is in a layer of the element for reaction with the analyte.

Multilayer analytical element containing crosslinked binder and method

A biologically active reagent is prepared by reacting a biologically active substance covalently to a polymeric particle having pendant aldehyde groups. Such groups are provided by a polymerizable monomer represented by the structure: ##STR1## wherein Ar is arylene, R and R.sup.1 are independently hydrogen, halo or lower alkyl, R.sup.2 and R.sup.3 are independently alkylene of 1 to 4 carbon atoms in the chain, R.sup.4 is arylene, m is 0 or 1, and n is an integer of 1 to 4. These reagents can be used in a variety of specific binding and affinity purification methods, and in both solution assays and dry analytical elements.

Biologically active reagent prepared from aldehyde-containing polymer,

New carbamazepine hapten analogues are described comprising: (A) an active ester group; (B) a carbamazepine nucleus; and (C) a linking chain (i) linking the carboxamide group of the carbamazepine nucleus to the active ester group, said linking chain having about 4 to about 40 atoms consisting of:

Carbamazepine hapten analogues

An element has been prepared which is useful for the detection of nucleic acids in various formats. The element has a sealable support on which is disposed a nucleic acid reagent composition. The composition is a mixture of a nucleic acid reagent composed of polymeric particles to which an oligonucleotide is covalently attached. The particles are prepared from a first polymer having a glass transition temperature of at least about 70.degree. C. and have an average diameter of from about 0.1 to about 3 micrometers. The reagent is adhered to the support using a water insoluble adhesive comprising a second polymer which has a glass transition temperature which is at least about 30.degree. C. less than the glass transition temperature of the first polymer. The adhesive is present in the composition at from about 1 to about 20 weight percent. This element provides high sensitivity and low background in hybridization and other nucleic acid assays.

Element and method for nucleic acid amplification and detection using

Biologically active reagents are prepared from particles of copolymers having highly active succinimid groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through amide groups by displacement of highly active succinimid groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods such as affinity chromatography.

Method of preparing biologically active reagents from

Labeled drug hapten analogues for immunoassays

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Pittsford, NY, United States of America

Ignazio S Ponticello