University of North Texas

Texas Christian University

Ignacy Gryczynski

Zygmunt Gryczynski

Julian Borejdo

Nils Calander

Badri P Maliwal

Rafal Fudala

Ryan M Rich

Rafal Luchowski

Evgenia Matveeva

The present invention includes a method and system for enhancing the signal-to-noise ratio in emission detection comprising: selecting a probe capable of at least one of fluorescence, phosphorescence, or delayed fluorescence in or about a sample that comprises interfering background signal; and exposing the probe to one or more controllable bursts, each burst comprising two or more pulses, wherein the one or more controllable bursts of high repetition energy pulses enhance the signal from the probe above that of the background signal.

Multiple-pulse pumping for enhanced fluorescence detection and molecular imaging in cells and tissue

The present invention includes compositions and methods for fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising: a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme.

FRET based multiplex probes

The present invention provides partially fluorescent nanoparticles and methods of making and using the partially fluorescent nanoparticle having a nanoparticle with a matrix and a fluorescent dye dispersed in or about the matrix, wherein at least a portion of the fluorescent dye has been anisotropically bleached.

Polarized fluorescent nanospheres

The present invention includes methods for ratiometric detection of analytes by surface plasmon coupled emission detection that includes disposing a target on the metal layer of a surface plasmon resonance detection system; coupling a first analyte to a first fluorescent dye and a second analyte to a second fluorescent dye; contacting the first and second analytes to the target on the surface plasmon resonance detection system; and measuring the intensity of a first and a second surface plasmon resonance enhanced fluorescence emission ring, wherein the first and second rings, respectively, quantitatively represents the amount of first and second analyte within 50 nanometers of the metal surface.

Ratiometric surface plasmon coupled emission detector

The present invention includes a microscope and a method for using the microscope for single molecule with reduced photobleaching of a fluorophore () that includes a light translucent material (); a metal layer () disposed on the light translucent material (); a medium () disposed on the metal layer (), the medium () having one or more fluorophores () capable of binding a target analyte (e.g., inside a cell); a microscope positioned to observe the surface plasmon emissions from the one or more fluorophores () within 50 nanometers of the surface of the metal layer (); an excitation source capable of exciting the one or more fluorophores (), the excitation source positioned to strike the light translucent material () at a first angle; and a light detector () that selectively detects emitted light generated by excited fluorophores () at a second angle (), wherein light emitted by the one or more fluorophores () at the surface plasmon angle is detected through the microscope, such that single molecules may be detected without significantly degrading fluorophore () emissions.

Growing community of inventors

Fort Worth, TX, United States of America

Ignacy Gryczynski