Universidad De Zaragoza

Centro De Investigacion Biomedica En Red

Centro De Investigacion Biomedica En Red (ciber)

Instituto Tecnológico De Aragón

Consejo Superior De Investigaciones Cientificas

Universiteit Twente

Fundación Rioja Salud

Ignacio Ochoa Garrido

Remco John Wiegerink

Jorge Santolaria Mazo

Rosa Maria Monge Prieto

Guillermo Llamazares Prieto

Javier Orús Pontaque

José Manuel Rodriguez Fortún

Carlos Peribáñez Subirón

Jose Luis Calavia Calvo

Luis José Fernández Ledesma

María Virumbrales Muñoz

José María Ayuso Domínguez

Luis J Fernández Ledesma

Luis Javier Fernández Ledesma

Manuel Doblaré Castellano

Carlos Sánchez Somolinos

Luis José Fernandez Ledesma

Rosa Monge Prieto

Sonia Martínez Herrero

Alfredo Martínez Ramírez

Ignacio Larráyoz Roldán

Josune García Sanmartín

Laura Ochoa Callejero

The present invention relates to a chip equipped with a plurality of compact microfluidic valves with multiple inlets and outlets, actuated by means of a flexible membrane system. The chip preferably comprises a deformable sealing layer made of at least one flexible, elastic, and insulating material; a structure formed by a succession of one or several microstructured layers, wherein said structure comprises one or several micro-chambers, one or several microfluidic channels, and one or several fluidic inlets and outlets; and wherein said structure is installed on a base substrate. The invention likewise relates to a microfluidic device that comprises the aforementioned chip, to a method for the fabrication of the chip, and to the uses associated with the chip and the microfluidic device.

Microfluidic chip, microfluidic device, associated uses and methods

The present invention relates to a novel microfluidic device and a microfluidic system for culturing cell samples based on: one or more culture wells () forming cell housing volumes for three-dimensional cell culture; one or more capillary channels () for circulating cells and/or fluid media, said channels () being connected to the wells () as well perfusion means; and an inlet port () and an outlet port (') for seeding cells and/or for circulating fluid in said channel (), said ports (&#x2032;) being connected to the ends of each capillary channel (). The novel configuration of the ports (&#x2032;) and their corresponding channels () allows known microfluidic perfusion media to be improved, dispensing with the use of complex elements for injecting the fluid media, such as syringe pumps, for example. The invention also allows two-dimensional and three-dimensional culture studies to be performed simultaneously.

Device and microfluidic system for studying cell cultures

Growing community of inventors

Madrid, Spain

Ignacio Ochoa Garrido