Mitsui Chemicals, Inc.

Ichirou Ikeda

Tadashi Araki

Toshihiro Oikawa

Kiyoteru Nagahara

Hiroki Ishibashi

Kaori Matoishi

Yasushi Fukuiri

Yasuko Matsuba

Reiko Abe

Nobuyuki Fukazawa

Tomoyuki Ando

Takeshi Nakamura

Katsutoshi Tsuchiya

Hironori Komatsu

Kiyoshi Ito

Tamotsu Asano

Hitoki Miyake

Hideki Umetani

Junya Fujiwara

Kyoko Maruyama

Takahiro Yamauchi

Hirokazu Awano

The invention provides a method for producing a cytosine nucleoside compound from pentose-1-phosphate and cytosine or a derivative thereof using a nucleoside phosphorylase reactive to cytosine or a bacterium having the enzyme activity. The invention also provides a method for specifically reducing an activity to degrade the substrates or the product, resulting in efficient production of the cytosine nucleoside compound. According to the invention, little by-product is produced in producing cytonucleocide compounds.

Method for producing cytosine nucleoside compounds

A desired isomer is selectively prepared by phosphorolyzing and isomerizing an anomer mixture of a 1-phosphorylated saccharide derivative while crystallizing one of the isomers to displace the equilibrium. Furthermore, using the action of a nucleoside phosphorylase, a nucleoside is prepared from the 1-phosphorylated saccharide derivative obtained and a base with improved stereoselectivity and a higher yield. This process is an anomer-selective process for preparing a 1-phosphorylated saccharide derivative and a nucleoside.

Process for selectively producing 1-phosphorylated sugar derivative anomer and process for producing nucleoside

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Chiba, Japan

Ichirou Ikeda