Nikon Corporation

Oki Electric Industry Co., Ltd.

Nippon Hoso' Kyokai

Victor Company

Oki Electric

Ibidi Gmbh

Ichiro Sase

Yutaka Sasaki

Osamu Yamada

Katsuya Watanabe

Miroslav Svoboda

Tatsuo Fukui

Toshiya Okabe

Akio Yanagimachi

Shuji Toyoda

Hirohisa Shishikura

Ryosuke Komatsu

Yasutoshi Kaneko

Roman Zantl

Masafumi Mimura

Toshiaki Nihoshi

Takaaki Okamoto

Yasushi Ogihara

Yuji Imaizumi

Yoshitaro Nakano

Yuki Terui

Kohki Konishi

Johana Trojanova

Martin Sajdl

Martin Berger

Jiri Sonsky

Shigeharu Ueguri

Petr Gazak

Shuji Nikon Corporation Toyoda

A sample preparation method includes: irradiating a first region of a sample with light at a time t; irradiating a second region different from the first region with the light at a time tafter the time t; and fixing the sample at a time tafter the time t. A sample preparing apparatus includes: a light radiating unit that irradiates a first region of a sample with light at time tand irradiates a second region different from the first region with the light at a time tafter the time t; and a fixing unit that fixes the sample at a time tafter the time t

Sample preparation method and sample preparing apparatus

A microscope apparatus including: an illumination optical system that radiates activation light to activate some of fluorescent materials included in a sample and excitation light to excite at least some of the activated fluorescent materials; an image forming optical system that: has an objective lens and an astigmatic optical system that generates astigmatism to at least part of fluorescence from the fluorescent materials; and forms an image of the fluorescence; an image-capturing unit that captures an image formed by the image forming optical system; a drive unit that moves an image-capturing position in the sample along an optical axis-direction of the objective lens; and a control unit, wherein the control unit causes the image-capturing unit to capture images in a plurality of numbers of frames respectively at a first image-capturing position and at a second image-capturing position different from the first image-capturing position.

Microscope apparatus

An image processor includes an image generator configured to generate corresponding image data corresponding to first microscopic image data obtained under a first observation condition, based on second microscopic image data and third microscopic image data obtained under a second observation condition, and an image output unit configured to output the corresponding image data. The corresponding image data may be image data corresponding to a first focal plane from which the first microscopic image data are obtained, and wherein the second microscopic image data and the third microscopic image data may be image data on a second focal plane and a third focal plane, respectively, which are different from the first focal plane.

Image processing device, microscope system, image processing method, and program

An image processor including: an accepting unit that accepts designation of at least part of three dimensional image of a microscope, and an image generator that generates image data for displaying, on a display, a first magnified image which corresponds to the part of the three dimensional image designated and a second magnified image which corresponds to the part of the three dimensional image designated and which is different from the first magnified image.

Image processing device, microscope system, image processing method, and computer program for displaying magnified images from different observation directions

An image processor includes an accepting unit that accepts designation of at least part of a three dimensional microscope image data, and an image generator that generates three dimensional magnified image data based on the designation. In the image processor, the image generator may sequentially output a first magnified image based on at least part of data of the three dimensional magnified image data, and a second magnified image based on the at least part of data of the three dimensional magnified image data which has at least part of data different from the first magnified image.

A sample preparation method includes: irradiating a first region of a sample with light at a time t1; irradiating a second region different from the first region with the tight at a time t2 after the time t1; and fixing the sample at a time t3 after the time t2. A sample preparing apparatus includes: a light radiating unit that irradiates a first region of a sample with light at time t1 and irradiates a second region different from the first region with the light at a time t2 after the time t1; and a fixing unit that fixes the sample at a time t3 after the time t2.

Provided is a microscope including: an illumination optical system that irradiates a specimen including a fluorescent substance with illumination light; an observation optical system including an objective lens; an imager that takes an image formed by the observation optical system; an image processor that performs image processing by using a result of the image taking; and a controller that moves at least one of a stage and the objective lens in the optical axis direction of the objective lens.

Microscope, observation method, and storage medium

An analysis device for quantifying a state of a fluorescent image containing a plurality of bright spots comprises an area setting unit in which states of a plurality of bright spots contained in a plurality of areas set in the fluorescent image in accordance with positions of the plurality of bright spots are quantified as numerical values.

Analysis device, microscope device, analysis method, and program

A microscope apparatus comprises: a distribution measurement apparatus which&#x2014;with respect to an observation region wherein a sample is arranged which comprises a fluorescent material that is activated when irradiated with an activating light of a prescribed wavelength, and that emits fluorescence when irradiated in an activated condition with an exciting light of a wavelength that differs from that of the activating light&#x2014;obtains a fluorescent picture image by conducting irradiation with the exciting light, and measures a fluorescent intensity distribution of the observation region; an irradiation intensity setting apparatus which sets irradiation intensities of the activating light for respective portions of the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus which obtains a plurality of the fluorescent picture images by multiply repeating operations comprising an operation wherein the observation region is irradiated with the activating light at the irradiation intensities that have been set in the respective portions, and an operation wherein a fluorescent picture image is obtained by irradiating the observation region with the exciting light after the activating light irradiation, and which generates a sample picture image from the plurality of the fluorescent picture images.

Microscope apparatus and observation method

An reference light emitted from an LED for auto focus enters a glass cover, on which a sample is adhered to, via a half mirror to an objective lens. The reference light that entered the glass cover is reflected by the boundary surface to be reflected light, and this reflected light enters a dichroic mirror via an objective lens. A part transmits the reflected light and allows the light to enter the camera via the dichroic mirror to the lens. A user rotates a motor-operated mirror while viewing the image of the reference light captured by a camera, so as to shift the reference light image position on the boundary surface.

Auto focus apparatus for detecting a focal point with a setting section for shifting an irradiation position outside an observation field of an imaging section

A laboratory dish comprises an incubation container, containing cultured cells, for observation through a microscope, in which a partitioning member partitions an interior of the incubation container into a plurality of incubation chambers by substantially perpendicular walls. The partitioning member tightly fits to a bottom portion of the incubation container, thereby capable of retaining in separation a liquid inputted in each of the incubation chambers from liquids in the other incubation chambers. A cover member covers an upper portion of each of the incubation chambers partitioned by the partitioning member and tightly fitted to the partitioning member. A whole or part of the cover member is formed of a transparent member, and the upper portion of each incubation chamber is formed with an opening via which to input the liquid.

Incubation container

A focus detection apparatus is provided with a light sourcethat emanates light with a given wavelength range; a reflection memberthat reflects light emanated from the light sourceto lead to an object, reflects light from the light sourcereflected from the object, and transmits light from the objectwith at least two different wavelength ranges except the light reflected from the object; a photodetectordetecting the light from the light sourcereflected from the object; and a controllerdetecting a focus shift between the objective lensand the objectin the microscopebased on a signal detected by the photodetector, thereby providing a focus detection apparatus capable of limiting wavelength range of light for focus detection thereby able to use wider wavelength range for the microscope observation, and a microscope equipped therewith.

Focus detection apparatus, microscope

Providing an imaging apparatus and a microscope capable of taking two-dimensional images of a sample at a plurality of observation positions different in the optical axis direction at the same time. The apparatus includes an image-forming lensthat forms images of a sampleon a plurality of image-forming places; an optical-path-dividing memberthat divides an optical path from the same area in a plane perpendicular to an optical axis of the sampleso as to form the plurality of image-forming places; and an optical-path-length-changing memberthat is provided on at least one optical path between the plurality of image-forming places and the imaging lens

Imaging apparatus and microscope

An observation field of view of a microscope can be moved without moving or changing an objective lens and without varying position or state of a sample. A microscope optical system has a mirror that changes the direction of the optical path by reflection and is located in the optical path between an objective lens of the microscope and an image to be observed. The mirror is able to be tilted by changing the position of a reflecting surface of the mirror. Accordingly, the observation field of view is moved by tilting the mirror. In other words, the observation field of view can be moved without changing a positional relationship between the objective lens of the microscope and the sample.

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Yokohama, Japan

Ichiro Sase