Nippon Oil Company

Ichiro Kojima

Yasuo Fujiwara

Kenji Maruhashi

Yutaka Oguchi

Takashi Suzuki

Tetsuo Satoh

Noboru Endoh

Takahiro Mori

Tatsuo Kinoshita

Tetsuo Toraya

Toshimitsu Hayashi

Hiroshi Sato

Yusuke Takahata

Kyoko Hayashi

Mamoru Yamanishi

Kouji Komiya

An antiviral polysaccharide purified from an extract prepared by extracting Spirulina cells with hot water, having the following properties:

Antiviral polysaccharide

A novel hydrophobic vitamin B.sub.12 derivative represented by the formula: ##STR1## wherein R represents an alkyl group having 2 to 17 carbon atoms, and X represents a cyano group, a hydroxyl group, an alkyl group or a 5'-deoxyadenosyl group. Since the derivative is hydrophobic, it can be taken up by an organism by thermodynamic diffusion, a passive transport, without an active transport.

Hydrophobic vitamin B.sub.12 derivatives

A process for producing vitamin B.sub.12 by the fermentation technique which comprises cultivating a vitamin B.sub.12 -producing microorganism belonging to the genus Propionibacterium in a culture medium containing a carbon source and a nitrogen source, and collecting vitamin B.sub.12 accumulated in the cells of the microorganism; characterized in that

Process for producing vitamin B.sub.12 by the fermentation technique,

A process for separating porphyrins, which comprises

Process for separating porphyrins

A process for separating vitamin B.sub.12 in pure form, which comprises bringing a vitamin B.sub.12 -containing liquid containing impurities into contact with a resin selected from the group consisting of (A) a divinylbenzene/styrene copolymer having a mode pore diameter of at least about 200 A and a total pore volume of more than 0.6 ml/g, and (B) a copolymer of divinylbenzene/styrene/an unsaturated alkyl ester of an aromatic polycarboxylic acid represented by the formula ##STR1## wherein R represents a C.sub.3 -C.sub.10 unsaturated alkyl moiety having a carbon-carbon double bond and n is 2 or 3, said copolymer (B) having a surface area of at least about 700 m.sup.2 /g, to cause vitamin B.sub.12 to be adsorbed to said resin, and eluting the vitamin B.sub.12 with an eluent, and collecting the active eluate.

Process for purifying and separating vitamin B.sub.12

A process for producing uroporphyrin III, which comprises cultivating a uroporphyrin III-producing microorganism of the genus Arthrobacter in a culture medium containing a carbon source, a nitrogen source and a mineral, and recovering uroporphyrin III from the culture broth.

Process for producing uroporphyrin III

A process for separating and recovering coproporphyrin and uroporphyrin from a culture broth containing them, which comprises

Process for separating and recovering coproporphyrin and uroporphyrin

In a process for producing coproporphyrin III which comprises cultivating a coproporphyrin III-producing microorganism of the genus Arthrobacter in a culture medium containing a carbon source, a nitrogen source and a mineral and recovering coproporphyrin III from the culture broth, the improvement wherein the culture medium contains at least 0.1 g, per liter of the culture medium, of L-cystine, at leas 0.5 g, per liter of the culture medium, of Mg.sup.++, or both; and coproporphyrin III-producing microoganisms.

Process for producing coproporphyrin III

A process for fermentatively producing vitamin B.sub.12, which comprises cultivating a vitamin B.sub.12 -producing microorganism belonging to at least one of the genus Arthrobacter and the genus Propionibacterium in the presence of a pre-cultivation product of a vitamin B.sub.12 -producing microorganism belonging to at least the other genus, and separating vitamin B.sub.12 from the culture broth.

Process for fermentatively producing vitamin B.sub.12

A process for producing vitamin B.sub.12, which comprises cultivating a vitamin B.sub.12 -producing micro-organism of the genus Arthrobacter under aerobic conditions in a culture medium containing at least one compound selected from the group consisting of alcohols with 2 or 3 carbon atoms and ketones, and separating vitamin B.sub.12 from the culture broth.

A process for producing glucose isomerase which comprises cultivating a glucose isomerase-producing bacterium belonging to the genus Corynebacterium under aerobic conditions in a culture medium containing carbon sources and nitrogen sources and separating the glucose isomerase-containing cells from the culture broth, and a process for producing fructose which comprises contacting glucose in an aqueous medium with the glucose isomerase-containing cells of a bacterium belonging to the genus Corynebacterium or glucose isomerase separated therefrom.

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Yokosuka, Japan

Ichiro Kojima