Roslin Institute (Edinburgh)

Roslin Institute

Other

Biotechnology & Biological Sciences Research Council

The Minister of Agriculture Fisheries and Food

Rosiin Institute (Edinburgh)

Ian Wilmut

Keith Henry Stockman Campbell

Keith Henry Campbell

A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.

Mammalian cultured inner cell mass cell culture using a g1 cell as nuclear donor

A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more animals. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.

Mammalian cultured inner cell mass cell culture using a quiescent cell as nuclear

A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the Gor Gphase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor such as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.

Unactivated oocytes as cytoplast recipients for nonprimate mammalian and pig nuclear transfer

Unactivated oocytes in nuclear transfer to produce ungulates

Methods of ungulate nuclear transfer

Unactivated oocytes in nuclear transfer to produce cultured inner cell mass cells and ungulates

Serial nuclear transfer of ungulate embryos

Unactivated ungulate oocytes to produce a transgenic ungulate by nuclear transfer

Unactivated ungulate oocytes to produce a cloned ungulate by nuclear transfer

Ungulates produced by nuclear transfer of G1 cells

Cloning ungulates from a quiescent donor cell

Unactivated oocytes as cytoplast recipients of quiescent cell nuclei while maintaining correct ploidy

Growing community of inventors

Midlothian, United Kingdom

Ian Wilmut