Life Technologies Corporation

Affymetrix, Inc.

Surromed, Inc.

Becton Dickinson and Company Limited

Ppd Biomarker Discovery Sciences, LLC

Leland Stanford Junior University

Sicpa Holding Sa

Nanoplex Technologies, Inc.

Affymetrix Technologies, N.v.

Ian Walton

Louis John Dietz

Richard P Rava

Mark O Trulson

Scott M Norton

David Stern

Michael J Natan

Peter J Fiekowsky

Achim Karger

Stephen P Fodor

James L Winkler

John Dixon

Robert L Cobene

Alexander V Dukhovny

William E Doering

Dan Kline

Adam Sannicandro

Aaron B Kantor

Byron Stephen Lee

Chih-Hua Chung

Shalom Tsur

Stephen P A Fodor

Sanjiv Sam Gambhir

Michael Simon

S Jeffrey Rosner

Jonathan Wang

Alexander Khorlin

Peter Ma

Remy Cromer

Walter G Stonas

Shay Keren

Edward Robert Holland

Swati Goyal

Michael Wenz

David Guagliardo

Sharron Gaynor Penn

R Griffith Freeman

Gabriela Chakarova

Gabriela S Chakarova

Robert Cobene

Sharron Penn

R Freeman Griffith

Rebecca Louise Stoermer

According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

Purification chemistries and formats for Sanger DNA sequencing reactions on a micro-fluidics device

In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.

Apparatuses, systems and methods for sequencing using capillary electrophoresis

The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. The system can also comprise an injection mechanism configured to deliver sample to the cathode end of the capillary array, and a temperature control zone, wherein the temperature control zone is configured to control the temperature of the interior of the housing.

Capillary array cartridge for capillary electrophoresis systems

An inline spectroscopic reader having a light source, one or more optics heads, a spectrometer and a data processing system in digital communication with the spectrometer detector. The optics heads include transmission optics providing for the illumination of a target with light from the light source and detection optics providing for the collection of light from the target. Typically, the target is moving with respect to the optics head during spectroscopic interrogation. The spectroscopic reader is thus an inline reader well suited to provide spectrum based production or analytical decision making in real time as the target moves along a production or analysis line. Also disclosed are methods including the steps of illuminating a target with light from a light source; collecting light from the target; obtaining a digitized spectrum with a spectrometer; extracting information content from the digitized spectrum; and basing a contemporaneous process decision upon the information content.

Inline spectroscopic reader and methods

Methods of imaging a living host using Raman nanoparticles; methods of generating a true image of a living host having been administered Raman nanoparticles; methods of multiplex imaging of a living host using a plurality of Raman nanoparticles; methods of generating multimodality images by combining Raman images with other functional/anatomical images; labeled Raman nanoparticles; and the like, are provided.

Molecular imaging of living subjects using Raman spectroscopy and labeled Raman nanoparticles

Surface Enhanced Spectroscopy-Active Composite Nanoparticles (SACN) comprising: core/shell nanoparticles comprising nanoparticle cores covered with shells, wherein the cores and the shells may comprise the same or different materials; at least one Raman-active reporter molecule associated with said core/shell nanoparticle; and an SiOencapsulant which encapsulates the core/shell nanoparticle and the at least one Raman-active reporter molecule, are described.

Surface enhanced spectroscopy-active composite nanoparticles comprising Raman-active reporter molecules

An encapsulated surface enhanced Raman scattering (SERS) tag. The tag includes a metal core and an encapsulant, typically a glass encapsulant. The encapsulant is further derivatized with a polymer.

Polymer coated SERS nanotag

The present invention provides an improved integrated system for biological marker identification. The system uses Microvolume Laser Scanning Microscopy (MLSC) in order to measure patterns of expression of biological markers in biological fluids. The system includes improved instrumentation for performing MLSC, and also includes improved particle detection and analysis methods. The system further comprises an informatics architecture for the analysis of data obtained from the MLSC in tandem with other medical information.

System for microvolume laser scanning cytometry

Methods and instrumentation for performing charge coupled device (CCD)-based confocal spectroscopy with a laser spot array are provided. The methods and instruments of the invention are useful in any spectroscopic application, including, but not limited to, microscopy and microvolume laser scanning cytometry (MLSC).

Optical architectures for microvolume laser-scanning cytometers

A method is disclosed for the manufacture of colloidal rod particles as nanobarcodes. Template membranes for the deposition of materials are prepared using photolithographic techniques.

Method of manufacture of colloidal rod particles as nanobarcodes

A system and method for imaging a sample labeled with a material having a strong light scattering and reflecting properties are provided. A typical material having strong light scattering and reflecting properties is a metal colloid. The imaging system employs a light scattering and reflecting illumination technique. The sample can be imaged with reflection mode imaging along or with a combination of reflection mode and scatter mode imaging.

Apparatus and method for detecting samples labeled with material having strong light scattering properties, using reflection mode light and diffuse scattering

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Redwood City, CA, United States of America

Ian Walton