Prolume, Ltd.

Unitargeting Research As

Palm Research As

Ian Pryme

Hanne Ravneberg

Albert Tauler

Beate Stern

John Hesketh

Christine Gjerdrum

John Edward Hesketh

The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.

Protein expression system

The invention relates to a kit to be used for the identification of a secretion cassette, which in eukaryotic cells gives rise to a higher level of synthesis/secretion of a protein of interest, the coding sequence of which being flanked by the first and the second set of genetic elements, respectively, compared to the other secretion cassettes present in the kit containing the coding sequence at the corresponding position, as well as to a method wherein the kit is used and the use of the kit, whereby the best secretion cassette will be obtained.

Kit for the optimisation of protein synthesis/secretion

An orally ingestible preparation consisting essentially of Mistletoe lectin I (ML-I), Mistletoe lectin II (ML-II), and Mistletoe lectin III (ML-III), which specifically excludes the nausea-inducing compounds present in mistletoe extracts. The invention also provides for a method of producing said preparation, a method of using said preparation to produce pharmaceutical preparations, and a method for using said preparation in the treatment of patients suffering from cancer and auto immune diseases.

Orally ingestible preparation of mistletoe lectins and method

Mammalian signal peptides can be used to aid in the secretion of mammalian proteins that are normally not secreted. By inactivating signals normally present in the 3' UTRs of mRNAs encoding proteins that are normally not secreted from mammalian cells, the proportion of such mRNA molecules directed to free and cytoskeletal bound polysomes can be reduced.

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Bergen, Norway

Ian Pryme