Health Protection Agency

Dynport Vaccine Company LLC

Immunobiology Limited

Ian McEntee

Randall K Lapcevich

Juan Arroyo

Rossalyn Brehm

Barbara T Solow

Nigel Allison

George Jack

Philip Vincent

Michael Herbert

John Brehm

Camilo Colaco

John Brehra

Barbara T Dynport Vaccine Company Llc Solow

Juan Dynport Vaccine Company Llc Arroyo

Randall K Dynport Vaccine Company Llc Lapcevich

The present invention relates to methods for the production of heat shock protein complexes for use in vaccine compositions. In particular, there is provided a method for increasing the level and immunogenicity of heat shock protein complexes produced in cells by subjecting the cells to specific stress inducing stimuli. The invention further extends to the use of heat shock protein complexes produced according to the methods of the invention in the preparation of vaccine compositions for the prevention and treatment of infectious diseases and cancerous conditions.

Method for the production of protein complexes and vaccine compositions comprising the same

A polynucleotide sequence is provided comprising a nucleic acid sequence encoding recombinant Protective Antigen (rPA). Also provided are expression vectors and host cells comprising the polynucleotide sequence of the invention, and methods for producing rPA.

Preparation of protective antigen

A polynucleotide sequence is provided, comprising: a nucleic acid sequence having at least 75% identity to SEQ ID NO: 1, wherein said nucleic acid sequence encodes recombinantProtective Antigen (rPA); or a fragment of said nucleic acid sequence wherein said fragment encodes a fragment of recombinantProtective Antigen (rPA). Also provided are expression vectors and host cells comprising the polynucleotide sequence of the invention, and methods for producing rPA or a fragment thereof. The invention further provides antigenic compositions and corresponding methods and uses for inducing an immune response.

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Salisbury, United Kingdom

Ian McEntee