Guild Associates, Inc.

Ian J Molineux

David Alexander Schofield

The present disclosure relates to compositions, methods, systems and kits for the detection of microorganisms of thespecies including. The disclosure relates to recombinant phage operable to infect amicroorganism, the phage comprising a detectable reporter. Detection systems of the disclosure may comprise a phage operable to infect amicroorganism, and may comprise a reporter nucleic acid expressible upon infection of amicroorganism by the phage. The system may be operable to detect the expression of the reporter. A detectable reporter may comprise any gene having bioluminescent, colorimetric and/or visual detectability. For example, a detectable reporter may comprise one or more luxAB genes detectable by emission, enhancement and/or change in spectrum of bioluminescent light. Live and infectiousmicrobes may be detected by the compositions, methods, systems and kits described herein.

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Austin, TX, United States of America

Ian J Molineux