Other

The Nutrasweet Company

Biotechnology Research Development Corporation

Ian G Fotheringham

Paul Taylor

Jennifer L Ton

David C Demirjian

Badal C Saha

Francis Michael Racine

Nathan Wymer

Roberta K Yoshida

Gene E Kidman

Jennifer Nelms

Chris Higgins

Mark P Scollar

Yoshikiyo Sakaibara

Larry E Robinson

The invention provides biosynthetic routes to xylitol production that do not require pure-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.

Methods for production of xylitol in microorganisms

The present invention provides materials and methods for production of D-phenylalanine by recombinant expression in a microorganisms of a D-aminotransferase gene and optionally a dadX gene in optional combination with eliminating or reducing expression of L-transaminase genes.

Materials and methods for the production of D-phenylalanine

Disclosed are novel bacterial cells characterized by hypersecretion of an amino acid, wherein a DNA inversion gene has been incorporated into said bacterial cells. Also disclosed are methods of producing said bacterial cells and methods of producing amino acids from said bacterial cells.

Materials and methods for hypersecretion of amino acids

Recombinant cells and an improved method utilizing them is disclosed for the preparation of an optically pure, L-amino acid from its D and D,L isomer forms. The process utilizes cell cultures that possess high aminotransferase activity that exhibits moderate selectivity in the conversion of D and L amino acids to their respective 2-keto acids as well as absolute stereospecificity in the conversion of the 2-keto acids to the L isomer alone.

Racemic conversion of using a transaminase

Disclosed are DNA sequences bonding deletion, substitution and/or addition analogs of the E. coli enzyme, chorismate mutase/prephenate dehydratase (CMPD). Preferred expression products include [des-Gln.sup.307, des-Ala.sup.308, des-Gly.sup.309, des-Ala.sup.310 ]CMPD; [Leu.sup.306 ]CMPD; [des-Thr.sup.304, Lys.sup.305, des-Gln.sup.306 ]CMPD; and [Cys.sup.309 ]CMPD display enzymatic activity of the wild type enzyme but are more resistant to inhibition in the presence of phenylalanine.

DNA encoding PHE a feedback inhibition resistant enzyme analogues

Growing community of inventors

Wheeling, IL, United States of America

Ian G Fotheringham