Sun Pharmaceutical Industries (australia) Pty Ltd

University of York

University of Leeds

Glaxosmithkline Australia Pty Limited

Sun Pharmaceutical Industries Australia Limited

Ian Alexander Graham

Thilo Hans Winzer

Tracy Carol Walker

Thierry Tonon

Monika Bielecka

Filip Kaminski

Andrew King

Alison Baker

Stephen Slocombe

Tony Robert Larson

Fergus Meade

Tracey Carol Walker

This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode cytochrome P450 polypeptides from acultivar; uses in the production of noscapine and identification of poppy cultivars that include genes that comprise said nucleic acid molecules.

Plant cytochrome P450

The disclosure relates to delta () and delta () desaturases and their use in the modification of oil content in hemp.

Desaturase nucleic acids and polypeptides

Papaver somniferum cytochrome P450

The present disclosure relates to nucleic acids that encode polypeptides with cytochrome P450 activity involved in the biosynthesis of plant derived diterpenoids or diterpenes.

Diterpenoid synthesis

This disclosure relates to the isolation of a nucleic acid molecule[s] that encode a novel cytochrome P450 and an oxidoreductase from a] cultivar, transgenic cells transformed with said nucleic acid molecule and sequence variants thereof; and including methods for the production of intermediates in the production of morphinans.

Cytochrome P450 fusion protein

The disclosure relates to a nucleic acid molecule isolated from acultivar that produces the opiate alkaloid noscapine which comprises 10 genes involved in the biosynthesis of opiate alkaloids.

Biosynthesis of opiate alkaloids

This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode methyltransferase polypeptides from acultivar; and uses in the production of noscapine and identification of poppy cultivars that include the genes that include these nucleic acid molecules.

Methyltransferase nucleic acids and polypeptides

We describe transgenic cells transformed with nucleic acid molecules which encode enzymes with desaturase activity and the use of these cells and enzymes in biocatalysis.

Desaturase enzymes

This invention relates to nucleic acid molecules which comprise nucleic acid sequences which encode enzymes involved in the biosynthesis of n-3 fatty acids, particularly docosahexanoic acid (DHA), or variants thereof; polypeptides encoded by said nucleic acids; cells transfected with said nucleic acid sequences and products comprising said nucleic acid sequences, polypeptides and/or cells.

Transgenic plants expressing enzymes involved in fatty acid biosynthesis

A nucleic acid which encodes a peroxisomal fatty acid transporter, uses thereof and a method of genetic manipulation of peroxisomal fatty acid transport and/or Metabolism. The nucleic acid and its products are especially for use in regulation of peroxisomal fatty acid transport in plant and in controlling the spectrum of fatty acids which can be utilised by the plant.

Regulation of peroxisomal fatty acid transport in plants

The invention provides a method for measuring an acyl coenzyme A (acyl CoA) ester or esters in a sample, comprising the steps of: a) forming a reaction mixture comprising the sample to be tested and a derivatizing agent; b) allowing the sample and said derivatizing agent to react, so as to form a fluorescent derivative(s) of any acyl CoA ester(s) present in the sample; and c) determining a level of said fluorescent derivative(s). Further provided is an assay for determining the level of an acyl coenzyme A (acyl CoA) ester or esters in a test sample, comprising the steps: a) obtaining the test sample from an organism to be tested; b) forming a reaction mixture comprising the sample to be tested and a derivatizing agent; c) allowing the sample and said derivatizing agent to react, so as to form a fluorescent derivative(s) of any acyl CoA ester(s) present in the sample; d) determining a level of said fluorescent derivative(s) ester(s); and e) comparing the level of said fluorescent derivative(s) in the test sample with a level of said fluorescent derivative(s) in a normal sample, such that a significant difference in the level of said fluorescent derivative(s) between the test sample and normal sample may be predictive of a fatty acid oxidation disorder in said organism. Also disclosed is a kit for use with the assay of the present invention.

Growing community of inventors

York, United Kingdom

Ian Alexander Graham