The United States of America, As Represented by the Secretary, Department of Health and Human Services

Tel Aviv University

Teva Pharmaceutical Industries Limited

Iafa Keydar

George F Vande Woude

Donna Faletto

Ilan Tsarfaty

James H Resau

A method for predicting breast tumor metastasis entails determining the amount of met protein in tumor tissue relative to normal breast duct tissue.

Met proto-oncogene and a method for predicting breast cancer progression

An immunoassay for diagnosing and monitoring human breast cancer. The assay employs a monoclonal antibody which recognizes a human mammary tumor virus derived from the T47D clone-10 breast cancer cell line (HMTV). The monoclonal antibody is produced by the hybridoma cell line deposited with the American Type Culture Collection under ATCC Accession No. HB 8630. When the immunoassay is performed on a tissue sample from a subject, the sample is contacted with monoclonal antibody ATCC Accession No. HB 8630 so as to form an antibody-antigen complex between the monoclonal antibody and any HMTV antigens which may be present in the sample. This antibody-antigen complex is then detected employing a second detectable antibody specific to the first monoclonal antibody. The presence or absence of the complex indicates the presence or absence of breast cancer in the subject. When the immunoassay is performed on a body fluid sample from a subject, the sample is first contacted with a detectable soluble antibody so as to form an antibody-antigen complex between the antibody and any HMTV antigens which may be present in the sample. The fluid sample is then contacted by a second antibody which is attached to a solid support. Either the first or second antibody is the monoclonal antibody produced by hybridoma cell line ATCC Accession No. HB 8630. The presence or absence of the complex indicates the presence or absence of human breast cancer in the subject.

Immunoassay for breast cancer employing monoclonal antibodies

Growing community of inventors

Tel Aviv, Israel

Iafa Keydar