Caliper Life Sciences, Inc.

Perkinelmer Health Sciences, Inc.

I-Jane Chen

Jingjing Wang

Laurel Provencher

Bradley W Rice

Daniel G Stearns

Tobias Daniel Wheeler

Joshua I Molho

Danh C Tran

Jun Yan

Andrew Barry

Seth Cohen

Wael I Yared

Alexander V Dukhovny

David Weinberger

A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.

Method and system of microfluidic immunoassay using magnetic beads

The invention provides microfluidic devices, systems, and methods for manipulating an object within a channel of a microfluidic device using an external electrode. The device has a channel disposed within the device, the channel having no included electrodes. The channel has a wall, at least a portion of which is penetrable by an electric field generated external to the device, the wall being penetrable such that the electric field extends through the wall portion and into a region within the channel. The system includes the microfluidic device and an electrode external to and not bonded to the device. In the method, the external electrode is placed adjacent to the device and energized to generate an electric field that extends through the wall of the device and into the channel, thereby manipulating an object within the channel.

Manipulation of objects in microfluidic devices using external electrodes

Methods and systems for reducing the run-to-run variability in measurement results obtained by a sample analysis system. The method and system utilize data from previous sample runs to optimize analysis parameters for future test runs. The methods and systems are particularly suitable for microfluidic sample analysis.

Method and system for microfluidic sample analysis

The invention provides devices, systems, and methods for extracting target objects from a sample. In the method, a stream of sample containing a plurality of target and non-target objects is directed by first and second streams of buffer through a sample inlet channel into a fluid junction and through the fluid junction into a sample waste channel. In response to detecting a target object within the stream of sample, an actuator is energized to close a normally open valve, resulting in a transient burst of cross-flow into the fluid junction that briefly diverts the flow of sample within the fluid junction and results in an aliquot of sample being directed into an aliquot delivery channel. The combination of the valve and the actuator acts as a self-limiting pulse generator.

Device and method for extracting target objects from a sample

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Alameda, CA, United States of America

I-Jane Chen