Regeneron Pharmaceuticals, Inc.

I-Hsuan Chen

Ning Li

Hui Xiao

Method of identifying host-cell proteins in a sample matrix are provided.

Identification of host cell proteins

The present application provides methods and systems to identify host cell protein (HCP) impurities in a sample containing high-abundance proteins. The HCP impurities can be enriched using interacting peptide ligands which have been attached to solid support. The HCP impurities can be eluted from the solid support using solution containing phase transfer surfactants. The isolated HCP impurities can be digested to generate components of the isolated HCP impurities which can subsequently be identified using a mass spectrometer.

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Redwood City, CA, United States of America

I-Hsuan Chen