Ge Healthcare Bio-sciences Ab

Biacore Ab

Håkan Roos

Magnus Malmqvist

Robert Karlsson

Mattias Tidare

Håkan Sjödin

Stefan Sjölander

Kjell Magnusson

Ralph Stålberg

Markku HÄMÄLÄINEN

Methods and devices are provided for controlling a fluid flow over a sensing surface within a flow cell. The methods employ laminar flow techniques to position a fluid flow over one or more discrete sensing areas on the sensing surface of the flow cell. Such methods permit selective sensitization of the discrete sensing areas, and provide selective contact of the discrete sensing areas with a sample fluid flow. Immobilization of a ligand upon the discrete sensing area, followed by selective contact with an analyte contained within the sample fluid flow, allows analysis by a wide variety of techniques. Sensitized sensing surfaces, and sensor devices and systems are also provided.

Method and device for laminar flow on a sensing surface

A method of operating an analytical flow cell device comprising an elongate flow cell having a first end and a second end, at least two ports at the first end and at least one port at the second end, comprises introducing a laminar flow of a first fluid at the first end of the flow cell, and a laminar counter flow of a second fluid at the second end. Each fluid flow is discharged at the first end or the second end, and the position of the interface between the first and second fluids in the longitudinal direction of the flow cell is adjusted by controlling the relative flow rates of the first and second fluids. Also disclosed are a method of analyzing a fluid sample for an analyte, a method of sensitizing a sensing surface, and a method of contacting a sensing surface with a test fluid.

Flow cell method

Growing community of inventors

Uppsala, Sweden

Håkan Roos