The University of Tokyo

Kawasaki Institute of Industrial Promotion

HyunJin Kim

Kanjiro Miyata

Kazunori Kataoka

Nobuhiro Nishiyama

Atsushi Ishii

Takehiko Ishii

Yasuki Kato

Hiroyasu Takemoto

Shigeto Fukushima

Makoto Oba

Sumiyo Watanabe

Kazuko Toh

Shourong Wu

Kotaro Hayashi

Yan Lee

Shigehiro Hiki

Mai Sanjo

Kanjiiro Miyata

An amphiphilic poly(amino acid) for nucleic acid delivery, which is represented by the following formula (1):

Amphiphilic poly (amino acid), block copolyer using the amphiphilic poly (amino acid), and complex including the amphiphilic poly (amino acid) or the block copolymer and nucleic acid

A unit-type polyion complex for use in delivering nucleic acid to a target site in a patient includes one or more molecules of a block copolymer having a poly(ethylene glycol) segment and a cationic poly(amino acid) segment and one or more molecules of a nucleic acid. A total quantity of positive charges derived from side chains of the cationic poly(amino acid) segment of the block copolymer in the unit-type polyion complex is not offset by a total quantity of negative charges derived from the nucleic acid. Furthermore, the nucleic acid has a strand length of 10-50 bases, the molecular weight of the poly(ethylene glycol) segment is 40&#xd7;10or more, and the block copolymer has a binding constant (Ka) for the nucleic acid of 3.0&#xd7;10or more.

Nucleic acid-loaded unit polyion complex

Disclosed is a polymer composite (polyplex) that contains nucleic acid, a cationic polymer, and an anionic polymer. The anionic polymer covers the surface of the composite comprising the cationic polymer and nucleic acid, has a negative charge at neutral pH, and can change so as to have a positive charge at mildly acidic pH.

Charge conversional ternary polyplex

The present invention provides an efficient delivery system for a nucleic acid, more specifically, a cationic poly(amino acid) that has a side chain having a plurality of different amine functional groups in a moiety including a cationic group and that has a hydrophobic group introduced into part of the side chain, and a polyion complex (PIC) of the poly(amino acid) and an oligo- or polynucleotide.

Cationic poly (amino acids) and uses thereof

The present invention relates to a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polyamino acid chain block, wherein the hydrophilic polymer chain block is covalently bound to one end of the main chain of the polyamino acid chain block, and the hydrophobic group is covalently bound to the side chains of not less than 10% and not greater than 70% of amino acid repeating units in the polyamino acid chain block. This block copolymer forms a stable aggregate with siRNA, a small-molecule nucleic acid, under a physiological condition.

Copolymer including uncharged hydrophilic block and cationic polyamino acid block having hydrophobic group in part of side chains, and use thereof

Growing community of inventors

Tokyo, Japan

HyunJin Kim