Korea Institute of Ocean Science & Technology

Korea Ocean Research and Development Institute

Korean Ocean Research & Development Institute

Hyun Sook Lee

Kae Kyoung Kwon

Sung Gyun Kang

Jung Hyun Lee

Yun Jae Kim

Sang Jin Kim

Jung Ho Jeon

Jae Kyu Lim

Seung Seop Bae

In Soon Jeong

Sun Shin Cha

Yona Cho

Tae Wan Kim

Min Sik Kim

Seung Seob Bae

Yun Ju Kim

Jeong Ho Jeon

Jung-Hyun Lee

Sung Hyun Yang

Seong Hyuk Lee

Ae Ran Choi

Suk Tae Kwon

Ji-in Yang

Jung Ho Hyun

Ki Hoon Nam

The present invention relates to an Fe—S fusion protein acting as an electron transport chain, a novel carbon monoxide:formate oxidoreductase (CFOR) including the Fe—S fusion protein, novelstrain BCF12 transformed with CFOR, and the use thereof. According to the present invention, two different enzymes may be physically linked directly to each other through the Fe—S fusion protein of the present invention, and thus electrons generated from any one enzyme may be transported directly to another enzyme through the Fe—S cluster of the Fe—S fusion protein. Accordingly, a reaction that produces a target substance with high efficiency by directly supplying electrons necessary for the production of the target substance is possible without leakage of electrons generated in any one enzyme. In addition, the present invention has an advantage in that the overall enzyme reaction rate and yield can be dramatically improved using a new electron transport reaction. Furthermore, it is possible to ensure the stability of each enzyme by allowing the enzymes to exist in a physically fixed state in cells.

Fe-S fusion protein acting as electron transfer chain, carbon monoxide formate redox enzyme mediated through FES fusion protein, strain BCF12 derived fromwherein enzyme is transformed, and use thereof

The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging tospp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.

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The present invention providesMC02 strain (accession no. KCTC12511BP) having increased hydrogen production ability, wherein the expression of rchA gene of the strain increases. Also, the present invention provides a method for producing the strain belonged to the genushaving increased hydrogen production ability comprising, increasing the expression of rchA gene of the strain, and hydrogen production method using the strain.

Thermococcus onnurineus MC02 and method of hydrogen production using thereof

The present invention relates to aNA1 mutant having an increased ability to produce hydrogen from formate and a method of producing hydrogen the same. TheNA1 mutant according to the invention has an increased ability to produce hydrogen in a formate-containing medium compared to wild-typeNA1 and shows an increase in growth rate compared to the wild-type. The use of the mutant strain according to the invention can produce hydrogen with high efficiency from formate.

The present invention relates to a hyperthermophilic DNA polymerase and a preparation method thereof. The invention provides a novel hyperthermophilic DNA polymerase isolated from asp. strain, a functional equivalent thereof, a protein having the amino acid sequence thereof, and a preparation method thereof. The DNA polymerase according to the invention is a DNA polymerase, which is hyperthermophilic and has an elongation ability and fidelity higher than those of prior commercial DNA polymerases. Thus, the DNA polymerase according to the invention will be useful in precision analysis, precision diagnosis, identification and the like, which require accurate PCR.

Hyperthermophilic DNA polymerase and methods of preparation thereof

Growing community of inventors

Gyeonggi-do, South Korea

Hyun Sook Lee