Korea Atomic Energy Research Institute

Hyo Kook Park

In Gyu Kim

Kug Chan Kim

Il Lae Jung

Byung Ho Choi

The present invention relates to a recombinanthaving phbCAB originated fromand an overproduction method of polyhydroxyalkanoate using the same, more precisely, a recombinant'MG1655/pTZ18U-PHB' or 'JIL938/pTZ18U-PHB' having phbCAB originated fromand an overproduction method of polyhydroxyalkanoate comprising the steps of preparing transformedhaving phbCAB originated from, inoculating and culturing the cells (growth phase), inducing the production of polyhydroxyalkanoate in the recomibnant(stationary phase and producing phase), and inducing the extracellular secretion of the polyhydroxyalkanoate from the recombinant. The method of the present invention facilitates not only overproduction of polyhydroxyalkanoate such as polyhydroxybutyrate by a simple batch culture but also industrial use of biodegradable polyhydroxyalkanoate replacing conventional non-biodegradable plastics by its simple and easy fermentation, separation and purification process.

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Taejon, South Korea

Hyo Kook Park