Korea Research Institute of Bioscience and Biotechnology

Hyeok Won Lee

Eun Gyo Lee

Joon Ki Jung

Jung Oh Ahn

Hong Weon Lee

Chun Sug Kim

Eui Sung Choi

Hong-Weon Lee

Ji Yeon Hong

Myoung Soo Park

Chun Suk Kim

Seung Hee Kang

Joo Hwan Lee

Jin Gyeom Lee

Jungoh Ahn

Yeon-Gu Kim

Shin Young Kang

Sun-Joo Park

Hee-Ju Ko

The present invention relates to a method for preparing a recombinant microorganism simultaneously comprising genes encoding enzymes used in the biosynthesis pathway of 6-aminocaproic acid, which is a precursor of caprolactam, biosynthesizing 6-aminocaproic acid from the microorganism, and producing the same so as to synthesize caprolactam.

Biological synthesis of 6-aminocaproic acid and transgenic microorganism therefor

The present invention relates to a new gene fragment derived from Chinese hamster ovary (CHO) cell for enhancement of recombinant protein expression in animal cells and a use thereof. It has been found that using the vector comprising a gene fragment of the present invention enhances the expression of a target protein in animal cells. Accordingly, the vector comprising a gene fragment of the present invention could be usefully used in the production of biopharmaceuticals such as therapeutic antibodies, etc.

Vector comprising gene fragment for enhancement of recombinant protein expression and use thereof

Disclosed herein are a-derived auto-inducible NPS promoter; a recombinant expression vector carrying the promoter and a nucleotide sequence, the sequence being operably linked to the promoter and coding for a recombinant protein; a host cell transformed or transfected with the recombinant expression vector; and a method for producing a recombinant protein, comprising culturing the host cell to express the recombinant protein and isolating the protein. The NPS promoter allows a recombinant protein of interest to be produced on a large scale without the need of any inducer.

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Disclosed are a-derived translation elongation factor (TEF) promoter, a recombinant expression vector carrying the promoter and a heterologous protein encoding base sequence operably linked to the promoter, and a host cell transfected or transformed with the recombinant expression vector. Also, a method is provided for producing a heterologous protein. The method comprises culturing the host cell to express the-derived TEF promoter and isolating the promoter from the culture. The TEF promoter can be utilized for the mass production of heterologous proteins without the need for inducers.

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Daejeon, South Korea

Hyeok Won Lee