Quest Diagnostics Investments, Inc.

Huong Mai

Michelle M Tabb

Maurice Exner

Michael Aye

Luca Jacky

Yin-Peng Chen

Lakshmi Nair

Heather Vincent

Jules Chen

Emberlee Eleazar

Provided herein are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids.

Direct amplification and detection of viral and bacterial pathogens

Disclosed are methods of identifying a methicillin-resistant(MRSA) in a sample wherein the methods involve detecting a-specific nucleic acid sequence, mecA and mecC, in the sample. Kits for determining the presence of MRSA in a sample are also provided.

Detection of methicillin-resistantin biological samples

Provided are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Further provided are reagent mixtures that allow for direct amplification of a sample, without the step of nucleic acid extraction.

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Irvine, CA, United States of America

Huong Mai