Academia Sinica

Academic Sinica

Hung-Ju Hsu

An-Suei Yang

Chung-Ming Yu

Hung-Pin Peng

Hong-Sen Chen

Yi-Kai Chiu

Wei-Ying Kuo

Yu-Ching Lee

Hwei-Jiung Wang

Ing-Chien Chen

Chao-Ping Tung

Keng-Chang Tsai

Yi-Jen Huang

Yueh-Liang Tsou

Hung-Ju Chang

Jhih-Wei Jian

Shih-Hsien Chuang

Michael Hsiao

Yu-Chi Chou

Shin-Chen Hou

Chao-Pin Lee

Ing-Chein Chen

Disclosed herein are methods for high-throughput screening of a functional antibody fragment for an immunoconjugate that targets a protein antigen. The method combines a phage-displayed synthetic antibody library and high-throughput cytotoxicity screening of non-covalently assembled immunotoxins or cytotoxic drug to identify highly functional synthetic antibody fragments for delivering toxin payloads.

High-throughput screening of functional antibody fragments, immunoconjugate comprising the same, and adaptor-drug conjugate for screening

Disclosed herein is an immunoconjugate comprising an antibody, a functional motif, and a linker connecting the functional motif to the antibody. According to embodiments of the present disclosure, the antibody may recognize tumor-associated antigens (TAAs), and serves as a targeting module for delivering the functional motif connected therewith to the tumor cells thereby inhibiting tumor growth or detecting the distribution of tumor cells. Also disclosed herein are methods of treating cancers and methods of diagnosing cancers by use of the present immunoconjugate.

Immunoconjugate and uses thereof

Disclosed herein is a phage-displayed single-chain variable fragment (scFv) library, which comprises a plurality of phage-displayed scFvs characterized in having a specific CS combination and a specific sequence in each CDR. The present scFv library is useful in efficiently producing different antibodies with binding affinity to different antigens. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.

Phage-displayed single-chain variable fragment libraries and uses thereof

Disclosed are nucleic acid libraries for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and for facilitating production of a disulfide-stabilized single chain antibody. Also disclosed are host cell libraries and phage libraries including the nucleic acid libraries. Further disclosed are methods for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and methods for producing a disulfide-stabilized single chain antibody and non-fusion form thereof.

Phage displaying system expressing single chain antibody

Growing community of inventors

Taipei, Taiwan

Hung-Ju Hsu