Industrial Technology Research Institute

Academia Sinica

Sino-american Silicon Products Inc.

Hung-Chi Chien

Chung-Fan Chiou

Chao-Chi Pan

Jenn-Yeh Fann

Hui-Ling Chen

Chih-Tsung Shih

Chang-Sheng Chu

Hui-Ping Tsai

Ming-Chia Li

Po-Ting Chen

Yi-Cheng Cheng

Tien-Soung Tong

Ying-Chih Pu

Ting-Shou Chen

Shu-Jiau Chiou

Chang-Jen Huang

Rita P-y Chen

Yung-Feng Liao

Cheng-Li Fang

Chein-Shiu Kuo

Bo-Kai Wu

Wen-Ching Hsu

Pei-Shin Jiang

Chih-Lung Lin

Yu-Yu Lin

Jiunn-Yih Chyan

Kun-Lin Yang

Rita P-Y Chen

Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.

Method of amplifying and determining target nucleotide sequence

Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.

Methods for accurate sequence data and modified base position determination

Disclosed herein is the novel use of use of aspp. extract, which may upregulate neprilysin, induce autophagy, protect neuron from amyloidopathy or tauopathy, and/or promote neurite outgrowth, thus thespp. extract of the present disclosure may be used as a dietary supplement for the prophylaxis or treatment of amyloid related neurodegenerative diseases so as to ameliorate or alleviate symptoms associated with the amyloid related neurodegenerative diseases.

extract and uses thereof

Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.

Single molecule detection system and methods

Single-molecule detection system and methods

The invention provides an epitaxial substrate, a semiconductor light-emitting device using such epitaxial substrate and fabrication thereof. The epitaxial substrate according to the invention includes a crystalline substrate. In particular, a crystal surface of the crystalline substrate thereon has a plurality of randomly arranged nanorods. The plurality of nanorods is formed of oxide of a material different from that forms the crystalline substrate.

Epitaxial substrate, semiconductor light-emitting device using such epitaxial substrate and fabrication thereof

Growing community of inventors

Hsinchu, Taiwan

Hung-Chi Chien